This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Green, K Y Articles by Dorsett, P H Search for Related Content PubMed PubMed Citation Articles by Green, K Y Articles by Dorsett, P H

 Previous Article  |  Next Article 

J Virol. 1986 March; 57(3): 893-898

Rubella virus antigens: localization of epitopes involved in hemagglutination and neutralization by using monoclonal antibodies.

ABSTRACT

Monoclonal antibodies (MAbs) against the rubella virion were used to locate epitopes involved in hemagglutination and neutralization. The MAbs exhibiting high-level hemagglutination-inhibiting activity were shown by Western blot analysis to be specific for the E1 polypeptide; this is consistent with the presence of the hemagglutinin on the E1 polypeptide. Some of the E1-specific MAbs also neutralized viral infectivity. However, hemagglutination-inhibiting activity and neutralizing activity did not always correlate. Three distinct functional epitopes were identified on the E1 polypeptide by competition analyses: one which reacted with MAbs with high-level hemagglutination-inhibiting activity and with neutralizing activity, one which reacted with MAbs with low-level hemagglutination-inhibiting activity and with neutralizing activity, and one which reacted with MAbs with only hemagglutination-inhibiting activity. A MAb specific for the E2 polypeptide exhibited neutralizing activity. This E2-specific MAb and two E1-specific MAbs with neutralizing activity were capable of precipitating intact virus which indicates that at least three epitopes involved in neutralization are accessible on the surface of the virion.

This article has been cited by other articles:

• Claus, C., Hofmann, J., Uberla, K., Liebert, U. G. (2006). Rubella virus pseudotypes and a cell-cell fusion assay as tools for functional analysis of the rubella virus E2 and E1 envelope glycoproteins.. J. Gen. Virol. 87: 3029-3037 [Abstract] [Full Text]  
• Cordoba, P., Lanoel, A., Grutadauria, S., Zapata, M. (2000). Evaluation of Antibodies against a Rubella Virus Neutralizing Domain for Determination of Immune Status. CVI 7: 964-966 [Abstract] [Full Text]  
• Lee, J.-Y., Bowden, D. S. (2000). Rubella Virus Replication and Links to Teratogenicity. Clin. Microbiol. Rev. 13: 571-587 [Abstract] [Full Text]  
• Bernasconi, E, Fasel, N, Wittek, R (1996). Cell surface expression of a functional rubella virus E1 glycoprotein by addition of a GPI anchor. J. Cell Sci. 109: 1195-1201 [Abstract]