JVI Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Weinberg, D H
Right arrow Articles by Ketner, G
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Weinberg, D H
Right arrow Articles by Ketner, G

 Previous Article  |  Next Article 

J Virol. 1986 March; 57(3): 833-838

Adenoviral early region 4 is required for efficient viral DNA replication and for late gene expression.

D H Weinberg and G Ketner

ABSTRACT

H2dl808 is a defective deletion mutant of human adenovirus 2 lacking most of transcriptional early region 4. Although the mutant can be grown in the complementing cell line W162, it is defective in human cell lines normally used to propagate adenovirus. In such nonpermissive cells, H2dl808 exhibits a severe defect in late gene expression, accumulating very small amounts of viral late messages and producing correspondingly small amounts of viral late proteins. H2dl808 also exhibits a defect in viral DNA synthesis: 24 h after infection, H2dl808-infected nonpermissive cells contain five- to sevenfold less viral DNA than those cells infected with wild-type adenovirus. H2dl808-infected nonpermissive cells eventually accumulate a significant amount of viral DNA. However, the rate of synthesis of viral proteins late in mutant infection remains much lower than that observed in wild-type infection at a time when DNA accumulation is comparable. Thus, the mutant's late protein synthesis defect is probably not due solely to its reduced accumulation of viral DNA. Finally, H2dl808 is much less efficient than wild-type virus in the inhibition of host cell protein synthesis in infections of nonpermissive cells. These observations imply roles for early region 4 products in several aspects of the viral growth cycle, including DNA replication, late gene expression, and host cell shutoff.

J Virol. 1986 March; 57(3): 833-838




This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Mol. Cell. Biol. Microbiol. Mol. Biol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 1986 by the American Society for Microbiology. All rights reserved.