This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Schiller, J T Articles by Lowy, D R Search for Related Content PubMed PubMed Citation Articles by Schiller, J T Articles by Lowy, D R

Next Article 

J Virol. 1986 January; 57(1): 1-6

E5 open reading frame of bovine papillomavirus type 1 encodes a transforming gene.

ABSTRACT

We have previously shown that the early region of the bovine papillomavirus type 1 genome contains two nonoverlapping segments that can independently induce the morphological transformation of cultured cells. The transforming gene from the 5' end of the early region is encoded by the E6 open reading frame. The second transforming segment was previously localized to a 2.3-kilobase fragment (2.3T) from the 3' end of the early region. To determine which of the four open reading frames (E2, E3, E4, and E5) located within 2.3T encodes a transforming gene, we have now introduced a series of insertion and deletion mutations into a clone (pHLB1) in which 2.3T is activated by the Harvey viral long terminal repeat, and we tested the mutants for their ability to induce focal transformation. Our results indicate that the E5 open reading frame, which could encode a low-molecular-weight hydrophobic peptide, is required for pHLB1-induced transformation of NIH 3T3 cells, but that the E2, E3, and E4 open reading frames are not.

This article has been cited by other articles:

• Petti, L. M., Ricciardi, E. C., Page, H. J., Porter, K. A. (2008). Transforming signals resulting from sustained activation of the PDGF{beta} receptor in mortal human fibroblasts. J. Cell Sci. 121: 1172-1182 [Abstract] [Full Text]  
• Hatama, S., Nobumoto, K., Kanno, T. (2008). Genomic and phylogenetic analysis of two novel bovine papillomaviruses, BPV-9 and BPV-10. J. Gen. Virol. 89: 158-163 [Abstract] [Full Text]  
• DeMasi, J., Chao, M. C., Kumar, A. S., Howley, P. M. (2007). Bovine Papillomavirus E7 Oncoprotein Inhibits Anoikis. J. Virol. 81: 9419-9425 [Abstract] [Full Text]  
• Ogawa, T., Tomita, Y., Okada, M., Shirasawa, H. (2007). Complete genome and phylogenetic position of bovine papillomavirus type 7. J. Gen. Virol. 88: 1934-1938 [Abstract] [Full Text]  
• DeMasi, J., Huh, K.-W., Nakatani, Y., Munger, K., Howley, P. M. (2005). Bovine papillomavirus E7 transformation function correlates with cellular p600 protein binding. Proc. Natl. Acad. Sci. USA 102: 11486-11491 [Abstract] [Full Text]  
• Fan, X., Liu, Y., Chen, J. J. (2003). Activation of c-Myc Contributes to Bovine Papillomavirus Type 1 E7-induced Cell Proliferation. J. Biol. Chem. 278: 43163-43168 [Abstract] [Full Text]  
• Fehrmann, F., Klumpp, D. J., Laimins, L. A. (2003). Human Papillomavirus Type 31 E5 Protein Supports Cell Cycle Progression and Activates Late Viral Functions upon Epithelial Differentiation. J. Virol. 77: 2819-2831 [Abstract] [Full Text]  
• Genther, S. M., Sterling, S., Duensing, S., Munger, K., Sattler, C., Lambert, P. F. (2003). Quantitative Role of the Human Papillomavirus Type 16 E5 Gene during the Productive Stage of the Viral Life Cycle. J. Virol. 77: 2832-2842 [Abstract] [Full Text]  
• Zhang, Y., Lehman, J. M., Petti, L. M. (2002). Apoptosis of Mortal Human Fibroblasts Transformed by the Bovine Papillomavirus E5 Oncoprotein. Mol Cancer Res 1: 122-136 [Abstract] [Full Text]  
• Nappi, V. M., Schaefer, J. A., Petti, L. M. (2002). Molecular Examination of the Transmembrane Requirements of the Platelet-derived Growth Factor beta Receptor for a Productive Interaction with the Bovine Papillomavirus E5 Oncoprotein. J. Biol. Chem. 277: 47149-47159 [Abstract] [Full Text]  
• Nappi, V. M., Petti, L. M. (2002). Multiple Transmembrane Amino Acid Requirements Suggest a Highly Specific Interaction between the Bovine Papillomavirus E5 Oncoprotein and the Platelet-Derived Growth Factor Beta Receptor. J. Virol. 76: 7976-7986 [Abstract] [Full Text]  
• Ashby, A. D. M., Meagher, L., Campo, M. S., Finbow, M. E. (2001). E5 transforming proteins of papillomaviruses do not disturb the activity of the vacuolar H+-ATPase. J. Gen. Virol. 82: 2353-2362 [Abstract] [Full Text]  
• Petti, L. M., Ray, F. A. (2000). Transformation of Mortal Human Fibroblasts and Activation of a Growth Inhibitory Pathway by the Bovine Papillomavirus E5 Oncoprotein. Cell Growth Differ. 11: 395-408 [Abstract] [Full Text]  
• Tong, X., Boll, W., Kirchhausen, T., Howley, P. M. (1998). Interaction of the Bovine Papillomavirus E6 Protein with the Clathrin Adaptor Complex AP-1. J. Virol. 72: 476-482 [Abstract] [Full Text]  
• Tong, X., Howley, P. M. (1997). The bovine papillomavirus E6 oncoprotein interacts with paxillin and disrupts the actin cytoskeleton. Proc. Natl. Acad. Sci. USA 94: 4412-4417 [Abstract] [Full Text]  
• Ghai, J., Ostrow, R. S., Tolar, J., McGlennen, R. C., Lemke, T. D., Tobolt, D., Liu, Z., Faras, A. J. (1996). The E5 gene product of rhesus papillomavirus is an activator of endogenous Ras and phosphatidylinositol-3'-kinase in NIH 3T3 cells. Proc. Natl. Acad. Sci. USA 93: 12879-12884 [Abstract] [Full Text]  
• Schlegel, R, Wade-Glass, M, Rabson, M., Yang, Y. (1986). The E5 transforming gene of bovine papillomavirus encodes a small, hydrophobic polypeptide. Science 233: 464-467 [Abstract]  
• Liu, Y., Hong, Y., Androphy, E. J., Chen, J. J. (2000). Rb-independent Induction of Apoptosis by Bovine Papillomavirus Type 1 E7 in Response to Tumor Necrosis Factor alpha. J. Biol. Chem. 275: 30894-30900 [Abstract] [Full Text]