This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Weir, J P Articles by Moss, B Search for Related Content PubMed PubMed Citation Articles by Weir, J P Articles by Moss, B

Use of a bacterial expression vector to identify the gene encoding a major core protein of vaccinia virus.

ABSTRACT

The DNA sequence of a vaccinia virus late gene contains an open reading frame that corresponds to the 28,000-dalton (28K) polypeptide made by in vitro translation of hybrid-selected mRNA. To further characterize the protein product of this late gene, we cloned a segment of DNA containing part of the open reading frame into a bacterial expression vector. The fusion protein produced from this vector, containing 151 amino acids of the predicted vaccinia virus protein, was used to immunize rabbits. The resulting antiserum specifically bound to a major 25K structural protein that is localized in the core of vaccinia virions, as well as to a 28K protein found in infected cells. Pulse-chase experiments indicated that the 25K core protein is originally made as a 28K precursor.

This article has been cited by other articles:

• Alzhanova, D., Hruby, D. E. (2006). A trans-Golgi Network Resident Protein, golgin-97, Accumulates in Viral Factories and Incorporates into Virions during Poxvirus Infection. J. Virol. 80: 11520-11527 [Abstract] [Full Text]  
• Ansarah-Sobrinho, C., Moss, B. (2004). Role of the I7 Protein in Proteolytic Processing of Vaccinia Virus Membrane and Core Components. J. Virol. 78: 6335-6343 [Abstract] [Full Text]