This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Irmiere, A Articles by Gibson, W Search for Related Content PubMed PubMed Citation Articles by Irmiere, A Articles by Gibson, W

 Previous Article  |  Next Article 

J Virol. 1985 October; 56(1): 277-283

Isolation of human cytomegalovirus intranuclear capsids, characterization of their protein constituents, and demonstration that the B-capsid assembly protein is also abundant in noninfectious enveloped particles.

ABSTRACT

Two types of intranuclear capsids have been recovered from human cytomegalovirus (HCMV, strain AD169)-infected cells. By analogy with strain Colburn (simian CMV) particles, these have been designated as A- and B-capsids. Both types of capsids are composed of proteins with molecular weights of 153,000 (major capsid protein), 34,000 (minor capsid protein), 28,000, and 11,000 (smallest capsid protein). In addition to these species, B-capsids contain a 36,000-molecular-weight (36K) protein which has been designated as the HCMV "assembly protein," based on its similarities to counterparts in strain Colburn CMV (i.e., 37K protein) and herpes simplex virus (i.e., VP22a/p40/NC-3/ICP35e). Peptide comparisons established that the assembly protein of HCMV B-capsids and the 36K protein that distinguishes HCMV noninfectious enveloped particles from virions are the same, providing direct evidence that noninfectious enveloped particles are enveloped B-capsids.

This article has been cited by other articles:

• Wang, D., Shenk, T. (2005). Human cytomegalovirus virion protein complex required for epithelial and endothelial cell tropism. Proc. Natl. Acad. Sci. USA 102: 18153-18158 [Abstract] [Full Text]  
• Loveland, A. N., Chan, C.-K., Brignole, E. J., Gibson, W. (2005). Cleavage of Human Cytomegalovirus Protease pUL80a at Internal and Cryptic Sites Is Not Essential but Enhances Infectivity. J. Virol. 79: 12961-12968 [Abstract] [Full Text]  
• Casaday, R. J., Bailey, J. R., Kalb, S. R., Brignole, E. J., Loveland, A. N., Cotter, R. J., Gibson, W. (2004). Assembly Protein Precursor (pUL80.5 Homolog) of Simian Cytomegalovirus Is Phosphorylated at a Glycogen Synthase Kinase 3 Site and Its Downstream "Priming" Site: Phosphorylation Affects Interactions of Protein with Itself and with Major Capsid Protein. J. Virol. 78: 13501-13511 [Abstract] [Full Text]  
• Adamo, J. E., Schroer, J., Shenk, T. (2004). Human Cytomegalovirus TRS1 Protein Is Required for Efficient Assembly of DNA-Containing Capsids. J. Virol. 78: 10221-10229 [Abstract] [Full Text]  
• Wang, S.-K., Duh, C.-Y., Wu, C.-W. (2004). Human Cytomegalovirus UL76 Encodes a Novel Virion-Associated Protein That Is Able To Inhibit Viral Replication. J. Virol. 78: 9750-9762 [Abstract] [Full Text]  
• Nixon, D. E., McVoy, M. A. (2004). Dramatic Effects of 2-Bromo-5,6-Dichloro-1-{beta}-D-Ribofuranosyl Benzimidazole Riboside on the Genome Structure, Packaging, and Egress of Guinea Pig Cytomegalovirus. J. Virol. 78: 1623-1635 [Abstract] [Full Text]  
• Lai, L., Britt, W. J. (2003). The Interaction between the Major Capsid Protein and the Smallest Capsid Protein of Human Cytomegalovirus Is Dependent on Two Linear Sequences in the Smallest Capsid Protein. J. Virol. 77: 2730-2735 [Abstract] [Full Text]  
• Chan, C.-K., Brignole, E. J., Gibson, W. (2002). Cytomegalovirus Assemblin (pUL80a): Cleavage at Internal Site Not Essential for Virus Growth; Proteinase Absent from Virions. J. Virol. 76: 8667-8674 [Abstract] [Full Text]  
• Stamminger, T., Gstaiger, M., Weinzierl, K., Lorz, K., Winkler, M., Schaffner, W. (2002). Open Reading Frame UL26 of Human Cytomegalovirus Encodes a Novel Tegument Protein That Contains a Strong Transcriptional Activation Domain. J. Virol. 76: 4836-4847 [Abstract] [Full Text]  
• Baxter, M. K., Gibson, W. (2001). Cytomegalovirus Basic Phosphoprotein (pUL32) Binds to Capsids In Vitro through Its Amino One-Third. J. Virol. 75: 6865-6873 [Abstract] [Full Text]  
• Plafker, S. M., Woods, A. S., Gibson, W. (1999). Phosphorylation of Simian Cytomegalovirus Assembly Protein Precursor (pAPNG.5) and Proteinase Precursor (pAPNG1): Multiple Attachment Sites Identified, Including Two Adjacent Serines in a Casein Kinase II Consensus Sequence. J. Virol. 73: 9053-9062 [Abstract] [Full Text]