This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Krug, R M
Right arrow Articles by Haller, O
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Krug, R M
Right arrow Articles by Haller, O

 Previous Article  |  Next Article 

J Virol. 1985 October; 56(1): 201-206

Inhibition of influenza viral mRNA synthesis in cells expressing the interferon-induced Mx gene product.

R M Krug, M Shaw, B Broni, G Shapiro and O Haller

ABSTRACT

Interferons alpha and beta induce an efficient antiviral state against influenza virus in mouse cells that possess the Mx gene, but not in mouse cells that lack this gene. In Mx-containing cells treated with interferon the amount of viral mRNA synthesized as a result of primary transcription is drastically reduced. Only two viral mRNAs could be detected by Northern analysis and by translating the poly(A)+ RNA from infected cells in wheat germ extracts: a reduced amount of the mRNA for nonstructural protein 1 and an even lower amount of the mRNA for the matrix protein. The other viral mRNAs were not made in detectable amounts. In addition, the rate of viral mRNA synthesis catalyzed by the inoculum transcriptase, measured by in vitro RNA synthesis catalyzed by permeabilized cells, was severely inhibited. In contrast, interferon treatment of cells lacking the Mx gene had little or no effect on either the steady-state level or the rate of synthesis of viral mRNAs made by the inoculum transcriptase. These results indicate that the interferon-induced Mx gene product, a 75,000-molecular-weight protein that accumulates in the nucleus, inhibits influenza viral mRNA synthesis which occurs in the nucleus. No Mx-specific effect acting directly on viral protein synthesis in the cytoplasm was observed.

J Virol. 1985 October; 56(1): 201-206




This article has been cited by other articles:

  • Benfield, C. T. O., Lyall, J. W., Kochs, G., Tiley, L. S. (2008). Asparagine 631 Variants of the Chicken Mx Protein Do Not Inhibit Influenza Virus Replication in Primary Chicken Embryo Fibroblasts or In Vitro Surrogate Assays. J. Virol. 82: 7533-7539 [Abstract] [Full Text]  
  • Dittmann, J., Stertz, S., Grimm, D., Steel, J., Garcia-Sastre, A., Haller, O., Kochs, G. (2008). Influenza A Virus Strains Differ in Sensitivity to the Antiviral Action of Mx-GTPase. J. Virol. 82: 3624-3631 [Abstract] [Full Text]  
  • Honda, A., Okamoto, T., Ishihama, A. (2007). Host factor Ebp1: Selective inhibitor of influenza virus transcriptase.. GENES CELLS 12: 133-142 [Abstract] [Full Text]  
  • Ottolini, M. G., Blanco, J. C. G., Eichelberger, M. C., Porter, D. D., Pletneva, L., Richardson, J. Y., Prince, G. A. (2005). The cotton rat provides a useful small-animal model for the study of influenza virus pathogenesis. J. Gen. Virol. 86: 2823-2830 [Abstract] [Full Text]  
  • Marcus, P. I., Rojek, J. M., Sekellick, M. J. (2005). Interferon Induction and/or Production and Its Suppression by Influenza A Viruses. J. Virol. 79: 2880-2890 [Abstract] [Full Text]  
  • Baskin, C. R., Garcia-Sastre, A., Tumpey, T. M., Bielefeldt-Ohmann, H., Carter, V. S., Nistal-Villan, E., Katze, M. G. (2004). Integration of Clinical Data, Pathology, and cDNA Microarrays in Influenza Virus-Infected Pigtailed Macaques (Macaca nemestrina). J. Virol. 78: 10420-10432 [Abstract] [Full Text]  
  • Turan, K., Mibayashi, M., Sugiyama, K., Saito, S., Numajiri, A., Nagata, K. (2004). Nuclear MxA proteins form a complex with influenza virus NP and inhibit the transcription of the engineered influenza virus genome. Nucleic Acids Res 32: 643-652 [Abstract] [Full Text]  
  • Seo, S. H., Webster, R. G. (2002). Tumor Necrosis Factor Alpha Exerts Powerful Anti-Influenza Virus Effects in Lung Epithelial Cells. J. Virol. 76: 1071-1076 [Abstract] [Full Text]  
  • Samuel, C. E. (2001). Antiviral Actions of Interferons. Clin. Microbiol. Rev. 14: 778-809 [Abstract] [Full Text]  
  • Miura, T. A., Carlson, J. O., Beaty, B. J., Bowen, R. A., Olson, K. E. (2001). Expression of Human MxA Protein in Mosquito Cells Interferes with LaCrosse Virus Replication. J. Virol. 75: 3001-3003 [Abstract] [Full Text]  
  • Gale, M. Jr., Tan, S.-L., Katze, M. G. (2000). Translational Control of Viral Gene Expression in Eukaryotes. Microbiol. Mol. Biol. Rev. 64: 239-280 [Abstract] [Full Text]  
  • Weber, F., Haller, O., Kochs, G. (2000). MxA GTPase Blocks Reporter Gene Expression of Reconstituted Thogoto Virus Ribonucleoprotein Complexes. J. Virol. 74: 560-563 [Abstract] [Full Text]  
  • Dessens, J. T., Nuttall, P. A. (1998). Mx1-Based Resistance to Thogoto Virus in A2G Mice Is Bypassed in Tick-Mediated Virus Delivery. J. Virol. 72: 8362-8364 [Abstract] [Full Text]  
  • Landis, H., Simon-Jodicke, A., Kloti, A., Di Paolo, C., Schnorr, J.-J., Schneider-Schaulies, S., Hefti, H. P., Pavlovic, J. (1998). Human MxA Protein Confers Resistance to Semliki Forest Virus and Inhibits the Amplification of a Semliki Forest Virus-Based Replicon in the Absence of Viral Structural Proteins. J. Virol. 72: 1516-1522 [Abstract] [Full Text]  
  • Nakata, T, Takemura, R, Hirokawa, N (1993). A novel member of the dynamin family of GTP-binding proteins is expressed specifically in the testis. J. Cell Sci. 105: 1-5 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»