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J Virol. 1985 September; 55(3): 533-546

Monoclonal antibodies specific for adenovirus early region 1A proteins: extensive heterogeneity in early region 1A products.

ABSTRACT

Hybridomas secreting monoclonal antibodies specific for the adenovirus early region 1A (E1A) proteins were prepared from BALB/c mice immunized with a bacterial trpE-E1A fusion protein. This protein is encoded by a hybrid gene that joins a portion of the Escherichia coli trpE gene and a cDNA copy of the E1A 13S mRNA (Spindler et al., J. Virol. 49:132-141, 1984). Eighty-three hybridomas that secrete antibodies which recognize the immunogen were isolated and single cell cloned. Twenty-nine of these antibodies are specific for the E1A portion of the fusion protein. Only 12 of the monoclonal antibodies can efficiently immunoprecipitate E1A polypeptides from detergent lysates of infected cells. E1A polypeptides were analyzed on one-dimensional, sodium dodecyl sulfate-polyacrylamide gels and two-dimensional, isoelectric focusing polyacrylamide gels. The E1A proteins that are specifically immunoprecipitated by the monoclonal antibodies are heterogeneous in size and charge and can be resolved into approximately 60 polypeptide species. This heterogeneity is due not only to synthesis from multiple E1A mRNAs, but also at least in part to post-translational modification. Several of the monoclonal antibodies divide the E1A polypeptides into immunological subclasses based on the ability of the antibodies to bind to the antigen. In particular, two of the monoclonal antibodies bind to the polypeptides synthesized from the 13S E1A mRNA, but not to other E1A proteins.

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• Johnson, J. S., Osheim, Y. N., Xue, Y., Emanuel, M. R., Lewis, P. W., Bankovich, A., Beyer, A. L., Engel, D. A. (2004). Adenovirus Protein VII Condenses DNA, Represses Transcription, and Associates with Transcriptional Activator E1A. J. Virol. 78: 6459-6468 [Abstract] [Full Text]  
• Toth, K., Djeha, H., Ying, B., Tollefson, A. E., Kuppuswamy, M., Doronin, K., Krajcsi, P., Lipinski, K., Wrighton, C. J., Wold, W. S. M. (2004). An Oncolytic Adenovirus Vector Combining Enhanced Cell-to-Cell Spreading, Mediated by the ADP Cytolytic Protein, with Selective Replication in Cancer Cells with Deregulated Wnt Signaling. Cancer Res. 64: 3638-3644 [Abstract] [Full Text]  
• Miura, T. A., Li, H., Morris, K., Ryan, S., Hembre, K., Cook, J. L., Routes, J. M. (2004). Expression of an E1A/E7 Chimeric Protein Sensitizes Tumor Cells to Killing by Activated Macrophages but Not NK Cells. J. Virol. 78: 4646-4654 [Abstract] [Full Text]  
• Chastain-Moore, A. M., Roberts, T., Trott, D. A., Newbold, R. F., Ornelles, D. A. (2003). An Activity Associated with Human Chromosome 21 Permits Nuclear Colocalization of the Adenovirus E1B-55K and E4orf6 Proteins and Promotes Viral Late Gene Expression. J. Virol. 77: 8087-8098 [Abstract] [Full Text]  
• Cook, J. L., Miura, T. A., Ikle, D. N., Lewis, A. M. Jr., Routes, J. M. (2003). E1A Oncogene-induced Sensitization of Human Tumor Cells to Innate Immune Defenses and Chemotherapy-induced Apoptosis in Vitro and in Vivo. Cancer Res. 63: 3435-3443 [Abstract] [Full Text]  
• De Luca, A., Mangiacasale, R., Severino, A., Malquori, L., Baldi, A., Palena, A., Mileo, A. M., Lavia, P., Paggi, M. G. (2003). E1A Deregulates the Centrosome Cycle in a Ran GTPase-dependent Manner. Cancer Res. 63: 1430-1437 [Abstract] [Full Text]  
• Madison, D. L., Yaciuk, P., Kwok, R. P. S., Lundblad, J. R. (2002). Acetylation of the Adenovirus-transforming Protein E1A Determines Nuclear Localization by Disrupting Association with Importin-alpha. J. Biol. Chem. 277: 38755-38763 [Abstract] [Full Text]  
• Wang, G., Berk, A. J. (2002). In Vivo Association of Adenovirus Large E1A Protein with the Human Mediator Complex in Adenovirus-Infected and -Transformed Cells. J. Virol. 76: 9186-9193 [Abstract] [Full Text]  
• Lafarga, M., Berciano, M. T., Pena, E., Mayo, I., Castano, J. G., Bohmann, D., Rodrigues, J. P., Tavanez, J. P., Carmo-Fonseca, M. (2002). Clastosome: A Subtype of Nuclear Body Enriched in 19S and 20S Proteasomes, Ubiquitin, and Protein Substrates of Proteasome. Mol. Biol. Cell 13: 2771-2782 [Abstract] [Full Text]  
• Martens, J. H. A., Verlaan, M., Kalkhoven, E., Dorsman, J. C., Zantema, A. (2002). Scaffold/Matrix Attachment Region Elements Interact with a p300-Scaffold Attachment Factor A Complex and Are Bound by Acetylated Nucleosomes. Mol. Cell. Biol. 22: 2598-2606 [Abstract] [Full Text]  
• Querido, E., Blanchette, P., Yan, Q., Kamura, T., Morrison, M., Boivin, D., Kaelin, W. G., Conaway, R. C., Conaway, J. W., Branton, P. E. (2001). Degradation of p53 by adenovirus E4orf6 and E1B55K proteins occurs via a novel mechanism involving a Cullin-containing complex. Genes Dev. 15: 3104-3117 [Abstract] [Full Text]  
• Chattopadhyay, D., Ghosh, M. K., Mal, A., Harter, M. L. (2001). Inactivation of p21 by E1A Leads to the Induction of Apoptosis in DNA-Damaged Cells. J. Virol. 75: 9844-9856 [Abstract] [Full Text]  
• Nevels, M., Täuber, B., Spruss, T., Wolf, H., Dobner, T. (2001). "Hit-and-Run" Transformation by Adenovirus Oncogenes. J. Virol. 75: 3089-3094 [Abstract] [Full Text]  
• Doronin, K., Kuppuswamy, M., Toth, K., Tollefson, A. E., Krajcsi, P., Krougliak, V., Wold, W. S. M. (2001). Tissue-Specific, Tumor-Selective, Replication-Competent Adenovirus Vector for Cancer Gene Therapy. J. Virol. 75: 3314-3324 [Abstract] [Full Text]  
• Zhang, Z., Smith, M. M., Mymryk, J. S. (2001). Interaction of the E1A Oncoprotein with Yak1p, a Novel Regulator of Yeast Pseudohyphal Differentiation, and Related Mammalian Kinases. Mol. Biol. Cell 12: 699-710 [Abstract] [Full Text]  
• Bordoli, L., Netsch, M., Luthi, U., Lutz, W., Eckner, R. (2001). Plant orthologs of p300/CBP: conservation of a core domain in metazoan p300/CBP acetyltransferase-related proteins. Nucleic Acids Res 29: 589-597 [Abstract] [Full Text]  
• Routes, J. M., Ryan, S., Clase, A., Miura, T., Kuhl, A., Potter, T. A., Cook, J. L. (2000). Adenovirus E1A Oncogene Expression in Tumor Cells Enhances Killing by TNF-Related Apoptosis-Inducing Ligand (TRAIL). J. Immunol. 165: 4522-4527 [Abstract] [Full Text]  
• Doronin, K., Toth, K., Kuppuswamy, M., Ward, P., Tollefson, A. E., Wold, W. S. M. (2000). Tumor-Specific, Replication-Competent Adenovirus Vectors Overexpressing the Adenovirus Death Protein. J. Virol. 74: 6147-6155 [Abstract] [Full Text]  
• Dick, F. A., Sailhamer, E., Dyson, N. J. (2000). Mutagenesis of the pRB Pocket Reveals that Cell Cycle Arrest Functions Are Separable from Binding to Viral Oncoproteins. Mol. Cell. Biol. 20: 3715-3727 [Abstract] [Full Text]  
• Mal, A., Chattopadhyay, D., Ghosh, M. K., Poon, R. Y.C., Hunter, T., Harter, M. L. (2000). p21 and Retinoblastoma Protein Control the Absence of DNA Replication in Terminally Differentiated Muscle Cells. J. Cell Biol. 149: 281-292 [Abstract] [Full Text]  
• Cenciarelli, C., De Santa, F., Puri, P. L., Mattei, E., Ricci, L., Bucci, F., Felsani, A., Caruso, M. (1999). Critical Role Played by Cyclin D3 in the MyoD-Mediated Arrest of Cell Cycle during Myoblast Differentiation. Mol. Cell. Biol. 19: 5203-5217 [Abstract] [Full Text]  
• Boivin, D., Morrison, M. R., Marcellus, R. C., Querido, E., Branton, P. E. (1999). Analysis of Synthesis, Stability, Phosphorylation, and Interacting Polypeptides of the 34-Kilodalton Product of Open Reading Frame 6 of the Early Region 4 Protein of Human Adenovirus Type 5. J. Virol. 73: 1245-1253 [Abstract] [Full Text]  
• Nevels, M., Täuber, B., Kremmer, E., Spruss, T., Wolf, H., Dobner, T. (1999). Transforming Potential of the Adenovirus Type 5 E4orf3 Protein. J. Virol. 73: 1591-1600 [Abstract] [Full Text]  
• Hateboer, G., Wobst, A., Petersen, B. O., Le Cam, L., Vigo, E., Sardet, C., Helin, K. (1998). Cell Cycle-Regulated Expression of Mammalian CDC6 Is Dependent on E2F. Mol. Cell. Biol. 18: 6679-6697 [Abstract] [Full Text]  
• Schoenberger, S. P., van der Voort, E. I. H., Krietemeijer, G. M., Offringa, R., Melief, C. J. M., Toes, R. E. M. (1998). Cutting Edge: Cross-Priming of CTL Responses In Vivo Does Not Require Antigenic Peptides in the Endoplasmic Reticulum of Immunizing Cells. J. Immunol. 161: 3808-3812 [Abstract] [Full Text]  
• Marcellus, R. C., Lavoie, J. N., Boivin, D., Shore, G. C., Ketner, G., Branton, P. E. (1998). The Early Region 4 orf4 Protein of Human Adenovirus Type 5 Induces p53-Independent Cell Death by Apoptosis. J. Virol. 72: 7144-7153 [Abstract] [Full Text]  
• Seghezzi, W., Chua, K., Shanahan, F., Gozani, O., Reed, R., Lees, E. (1998). Cyclin E Associates with Components of the Pre-mRNA Splicing Machinery in Mammalian Cells. Mol. Cell. Biol. 18: 4526-4536 [Abstract] [Full Text]  
• de Stanchina, E., McCurrach, M. E., Zindy, F., Shieh, S.-Y., Ferbeyre, G., Samuelson, A. V., Prives, C., Roussel, M. F., Sherr, C. J., Lowe, S. W. (1998). E1A signaling to p53 involves the p19ARF tumor suppressor. Genes Dev. 12: 2434-2442 [Abstract] [Full Text]  
• Karuppayil, S. M., Moran, E., Das, G. M. (1998). Differential Regulation of p53-dependent and -independent Proliferating Cell Nuclear Antigen Gene Transcription by 12 S E1A Oncoprotein Requires CBP. J. Biol. Chem. 273: 17303-17306 [Abstract] [Full Text]  
• Samuelson, A. V., Lowe, S. W. (1997). Selective induction of p53 and chemosensitivity in RB-deficient cells by E1A mutants unable to bind the RB-related proteins. Proc. Natl. Acad. Sci. USA 94: 12094-12099 [Abstract] [Full Text]  
• Bishopric, N. H., Zeng, G.-Q., Sato, B., Webster, K. A. (1997). Adenovirus E1A Inhibits Cardiac Myocyte-specific Gene Expression through Its Amino Terminus. J. Biol. Chem. 272: 20584-20594 [Abstract] [Full Text]  
• Helin, K., Holm, K., Niebuhr, A., Eiberg, H., Tommerup, N., Hougaard, S., Poulsen, H. S., Spang-Thomsen, M., Norgaard, P. (1997). Loss of the retinoblastoma protein-related p130 protein in small cell lung carcinoma. Proc. Natl. Acad. Sci. USA 94: 6933-6938 [Abstract] [Full Text]  
• Shen, Y., Zhu, H., Shenk, T. (1997). Human cytomegalovirus IE1 and IE2 proteins are mutagenic and mediate "hit-and-run" oncogenic transformation in cooperation with the adenovirus E1A proteins. Proc. Natl. Acad. Sci. USA 94: 3341-3345 [Abstract] [Full Text]  
• Mazars, G. R., Jat, P. S. (1997). Expression of p24, a novel p21Waf1/Cip1/Sdi1-related protein, correlates with measurement of the finite proliferative potential of rodent embryo fibroblasts. Proc. Natl. Acad. Sci. USA 94: 151-156 [Abstract] [Full Text]  
• Cook, J. L., Krantz, C. K., Routes, B. A. (1996). Role of p300-family proteins in E1A oncogene induction of cytolytic susceptibility and tumor cell rejection. Proc. Natl. Acad. Sci. USA 93: 13985-13990 [Abstract] [Full Text]  
• Lee, J.-S., Zhang, X., Shi, Y. (1996). Differential Interactions of the CREB/ATF Family of Transcription Factors with p300 and Adenovirus E1A. J. Biol. Chem. 271: 17666-17674 [Abstract] [Full Text]  
• Dynlacht, B D, Flores, O, Lees, J A, Harlow, E (1994). Differential regulation of E2F transactivation by cyclin/cdk2 complexes.. Genes Dev. 8: 1772-1786 [Abstract]  
• Eckner, R, Ewen, M E, Newsome, D, Gerdes, M, DeCaprio, J A, Lawrence, J B, Livingston, D M (1994). Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor.. Genes Dev. 8: 869-884 [Abstract]  
• Tsai, L. H., Takahashi, T., Caviness, V. S., Harlow, E. (1993). Activity and expression pattern of cyclin-dependent kinase 5 in the embryonic mouse nervous system. Development 119: 1029-1040 [Abstract]  
• Li, Y, Graham, C, Lacy, S, Duncan, A M, Whyte, P (1993). The adenovirus E1A-associated 130-kD protein is encoded by a member of the retinoblastoma gene family and physically interacts with cyclins A and E.. Genes Dev. 7: 2366-2377 [Abstract]  
• Cobrinik, D, Whyte, P, Peeper, D S, Jacks, T, Weinberg, R A (1993). Cell cycle-specific association of E2F with the p130 E1A-binding protein.. Genes Dev. 7: 2392-2404 [Abstract]  
• Helin, K, Wu, C L, Fattaey, A R, Lees, J A, Dynlacht, B D, Ngwu, C, Harlow, E (1993). Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation.. Genes Dev. 7: 1850-1861 [Abstract]  
• Boyer, T G, Berk, A J (1993). Functional interaction of adenovirus E1A with holo-TFIID.. Genes Dev. 7: 1810-1823 [Abstract]  
• Zhu, L, van den Heuvel, S, Helin, K, Fattaey, A, Ewen, M, Livingston, D, Dyson, N, Harlow, E (1993). Inhibition of cell proliferation by p107, a relative of the retinoblastoma protein.. Genes Dev. 7: 1111-1125 [Abstract]  
• Lowe, S W, Ruley, H E (1993). Stabilization of the p53 tumor suppressor is induced by adenovirus 5 E1A and accompanies apoptosis.. Genes Dev. 7: 535-545 [Abstract]  
• Lees, E, Faha, B, Dulic, V, Reed, S I, Harlow, E (1992). Cyclin E/cdk2 and cyclin A/cdk2 kinases associate with p107 and E2F in a temporally distinct manner.. Genes Dev. 6: 1874-1885 [Abstract]  
• Faha, B, Ewen, M., Tsai, L., Livingston, D., Harlow, E (1992). Interaction between human cyclin A and adenovirus E1A-associated p107 protein. Science 255: 87-90 [Abstract]  
• Giordano, A, Lee, J., Scheppler, J., Herrmann, C, Harlow, E, Deuschle, U, Beach, D, Franza, B. Jr (1991). Cell cycle regulation of histone H1 kinase activity associated with the adenoviral protein E1A. Science 253: 1271-1275 [Abstract]  
• Muller, U, Roberts, M P, Engel, D A, Doerfler, W, Shenk, T (1989). Induction of transcription factor AP-1 by adenovirus E1A protein and cAMP.. Genes Dev. 3: 1991-2002 [Abstract]  
• Huang, M M, Hearing, P (1989). The adenovirus early region 4 open reading frame 6/7 protein regulates the DNA binding activity of the cellular transcription factor, E2F, through a direct complex.. Genes Dev. 3: 1699-1710 [Abstract]  
• Dyson, N, Howley, P., Munger, K, Harlow, E (1989). The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product. Science 243: 934-937 [Abstract]  
• Horowitz, J., Yandell, D., Park, S., Canning, S, Whyte, P, Buchkovich, K, Harlow, E, Weinberg, R., Dryja, T. (1989). Point mutational inactivation of the retinoblastoma antioncogene. Science 243: 937-940 [Abstract]  
• Spangler, R, Bruner, M, Dalie, B, Harter, M. (1987). Activation of adenovirus promoters by the adenovirus E1A protein in cell-free extracts. Science 237: 1044-1046 [Abstract]  
• Sang, N., Severino, A., Russo, P., Baldi, A., Giordano, A., Mileo, A. M., Paggi, M. G., De Luca, A. (2001). RACK1 Interacts with E1A and Rescues E1A-induced Yeast Growth Inhibition and Mammalian Cell Apoptosis. J. Biol. Chem. 276: 27026-27033 [Abstract] [Full Text]