This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chatterjee, P K Articles by Flint, S J Search for Related Content PubMed PubMed Citation Articles by Chatterjee, P K Articles by Flint, S J

 Previous Article  |  Next Article 

J Virol. 1985 August; 55(2): 379-386

Interactions among the three adenovirus core proteins.

ABSTRACT

Interactions among the three adenovirus core polypeptides V, VII, and mu were examined, using the reversible chemical cross-linker dithiobis(succinimidyl propionate) and two-dimensional polyacrylamide gel electrophoresis. Cross-linked species obtained from gradient-purified adenovirus type 2 cores were well represented among the cross-linked products of pentonless virions and crude core preparations. The more efficiently formed cross-linked core species were also identified with the arginine-specific cross-linker, p-azidophenyl glyoxal. In addition to dimers of polypeptides V and VII, efficient cross-linking of V to VII, V to mu, and VII to V to mu was detected in adenovirus cores. Notably absent were cross-linked species corresponding to higher multimers of polypeptide VII. A major core-capsid interaction appeared to be via the association of polypeptide V with a dimer of polypeptide VI.

This article has been cited by other articles:

• Saban, S. D., Silvestry, M., Nemerow, G. R., Stewart, P. L. (2006). Visualization of {alpha}-Helices in a 6-Angstrom Resolution Cryoelectron Microscopy Structure of Adenovirus Allows Refinement of Capsid Protein Assignments. J. Virol. 80: 12049-12059 [Abstract] [Full Text]  
• Wodrich, H., Cassany, A., D'Angelo, M. A., Guan, T., Nemerow, G., Gerace, L. (2006). Adenovirus Core Protein pVII Is Translocated into the Nucleus by Multiple Import Receptor Pathways. J. Virol. 80: 9608-9618 [Abstract] [Full Text]  
• Haruki, H., Okuwaki, M., Miyagishi, M., Taira, K., Nagata, K. (2006). Involvement of Template-Activating Factor I/SET in Transcription of Adenovirus Early Genes as a Positive-Acting Factor. J. Virol. 80: 794-801 [Abstract] [Full Text]  
• Vellinga, J., Van der Heijdt, S., Hoeben, R. C. (2005). The adenovirus capsid: major progress in minor proteins. J. Gen. Virol. 86: 1581-1588 [Abstract] [Full Text]  
• Vellinga, J., Rabelink, M. J. W. E., Cramer, S. J., van den Wollenberg, D. J. M., Van der Meulen, H., Leppard, K. N., Fallaux, F. J., Hoeben, R. C. (2004). Spacers Increase the Accessibility of Peptide Ligands Linked to the Carboxyl Terminus of Adenovirus Minor Capsid Protein IX. J. Virol. 78: 3470-3479 [Abstract] [Full Text]  
• Lee, T. W. R., Lawrence, F. J., Dauksaite, V., Akusjarvi, G., Blair, G. E., Matthews, D. A. (2004). Precursor of human adenovirus core polypeptide Mu targets the nucleolus and modulates the expression of E2 proteins. J. Gen. Virol. 85: 185-196 [Abstract] [Full Text]  
• Lee, T. W. R., Blair, G. E., Matthews, D. A. (2003). Adenovirus core protein VII contains distinct sequences that mediate targeting to the nucleus and nucleolus, and colocalization with human chromosomes. J. Gen. Virol. 84: 3423-3428 [Abstract] [Full Text]  
• Matthews, D. A. (2001). Adenovirus Protein V Induces Redistribution of Nucleolin and B23 from Nucleolus to Cytoplasm. J. Virol. 75: 1031-1038 [Abstract] [Full Text]  
• Akalu, A., Liebermann, H., Bauer, U., Granzow, H., Seidel, W. (1999). The Subgenus-Specific C-Terminal Region of Protein IX Is Located on the Surface of the Adenovirus Capsid. J. Virol. 73: 6182-6187 [Abstract] [Full Text]