This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Saito, I Articles by Shimojo, H Search for Related Content PubMed PubMed Citation Articles by Saito, I Articles by Shimojo, H

Construction of nondefective adenovirus type 5 bearing a 2.8-kilobase hepatitis B virus DNA near the right end of its genome.

ABSTRACT

A novel helper-free adenovirus type 5 (Ad5) vector system, which utilizes a cloning site 0.2 kilobase (kb) from the right end of the genome, has been developed. To construct a nondefective Ad5 bearing the 2.8-kb DNA fragment of hepatitis B virus (HBV) at this site, we deleted the 2.1-kb nonessential E3 fragment from cloned DNA covering the right one-fourth of the Ad5 genome (76 to 100 map units), inserted the HBV DNA into this site, ligated the recombinant DNA to the rest of the Ad5 genome, and transfected the ligated DNA into human embryo kidney cells. Most of the recovered virus clones had only the E3 deletion and no HBV insertion, suggesting that a homologous recombination occurs between transfected DNAs in these cells. The isolated Ad5 virus bearing the HBV DNA (Ad5-HBL) grew without helper virus in HeLa cells as efficiently as wild-type Ad5, although the 1.9-kb major E4 transcript was detected only poorly in the early phase in the Ad5-HBL-infected cells, suggesting that the HBV DNA inserted upstream of the E4 promoter reduces the E4 transcript. HBV mRNAs transcribed from the inserted DNA were at least as abundant as Ad5 early mRNAs in the late phase of Ad5-HBL infection, but the HBV surface antigen was barely detectable in the infected-cell lysate and culture medium. This result suggests that HBV mRNAs can be transcribed from the inserted genes but no protein can be translated from the HBV mRNAs, presumably because of the translational suppression of cellular mRNAs caused by adenovirus in its late phase.

This article has been cited by other articles:

• Nakano, M., Odaka, K., Takahashi, Y., Ishimura, M., Saito, I., Kanegae, Y. (2005). Production of viral vectors using recombinase-mediated cassette exchange. Nucleic Acids Res 33: e76-e76 [Abstract] [Full Text]  
• Brederlau, A., Faigle, R., Elmi, M., Zarebski, A., Sjoberg, S., Fujii, M., Miyazono, K., Funa, K. (2004). The Bone Morphogenetic Protein Type Ib Receptor Is a Major Mediator of Glial Differentiation and Cell Survival in Adult Hippocampal Progenitor Cell Culture. Mol. Biol. Cell 15: 3863-3875 [Abstract] [Full Text]  
• Kuwabara, K., Nakaoka, T., Sato, K., Nishishita, T., Sasaki, T., Yamashita, N. (2004). Differential Regulation of Cell Migration and Proliferation through Proline-Rich Tyrosine Kinase 2 in Endothelial Cells. Endocrinology 145: 3324-3330 [Abstract] [Full Text]  
• Faigle, R., Brederlau, A., Elmi, M., Arvidsson, Y., Hamazaki, T. S., Uramoto, H., Funa, K. (2004). ASK1 Inhibits Astroglial Development via p38 Mitogen-Activated Protein Kinase and Promotes Neuronal Differentiation in Adult Hippocampus-Derived Progenitor Cells. Mol. Cell. Biol. 24: 280-293 [Abstract] [Full Text]  
• Loser, P., Hofmann, C., Both, G. W., Uckert, W., Hillgenberg, M. (2003). Construction, Rescue, and Characterization of Vectors Derived from Ovine Atadenovirus. J. Virol. 77: 11941-11951 [Abstract] [Full Text]  
• Kondo, S., Okuda, A., Sato, H., Tachikawa, N., Terashima, M., Kanegae, Y., Saito, I. (2003). Simultaneous on/off regulation of transgenes located on a mammalian chromosome with Cre-expressing adenovirus and a mutant loxP. Nucleic Acids Res 31: e76-e76 [Abstract] [Full Text]  
• Nakagawa, T., Abe, M., Yamazaki, T., Miyashita, H., Niwa, H., Kokubun, S., Sato, Y. (2003). HEX Acts as a Negative Regulator of Angiogenesis by Modulating the Expression of Angiogenesis-Related Gene in Endothelial Cells In Vitro. Arterioscler. Thromb. Vasc. Bio. 23: 231-237 [Abstract] [Full Text]  
• Inoshita, S., Takeda, K., Hatai, T., Terada, Y., Sano, M., Hata, J., Umezawa, A., Ichijo, H. (2002). Phosphorylation and Inactivation of Myeloid Cell Leukemia 1 by JNK in Response to Oxidative Stress. J. Biol. Chem. 277: 43730-43734 [Abstract] [Full Text]  
• Sato, S., Tatebayashi, Y., Akagi, T., Chui, D.-H., Murayama, M., Miyasaka, T., Planel, E., Tanemura, K., Sun, X., Hashikawa, T., Yoshioka, K., Ishiguro, K., Takashima, A. (2002). Aberrant Tau Phosphorylation by Glycogen Synthase Kinase-3beta and JNK3 Induces Oligomeric Tau Fibrils in COS-7 Cells. J. Biol. Chem. 277: 42060-42065 [Abstract] [Full Text]  
• YOKOO, T., OHASHI, T., UTSUNOMIYA, Y., SHIBA, H., SHEN, J. S., HISADA, Y., ETO, Y., KAWAMURA, T., HOSOYA, T. (2001). Inflamed Glomeruli--Specific Gene Activation that Uses Recombinant Adenovirus with the Cre/loxP System. J. Am. Soc. Nephrol. 12: 2330-2337 [Abstract] [Full Text]  
• Woods, J. M., Katschke, K. J. Jr., Volin, M. V., Ruth, J. H., Woodruff, D. C., Amin, M. A., Connors, M. A., Kurata, H., Arai, K.-I., Haines, G. K. III, Kumar, P., Koch, A. E. (2001). IL-4 Adenoviral Gene Therapy Reduces Inflammation, Proinflammatory Cytokines, Vascularization, and Bony Destruction in Rat Adjuvant-Induced Arthritis. J. Immunol. 166: 1214-1222 [Abstract] [Full Text]  
• Woods, J. M., Katschke, K. J. Jr., Tokuhira, M., Kurata, H., Arai, K.-I., Campbell, P. L., Koch, A. E. (2000). Reduction of Inflammatory Cytokines and Prostaglandin E2 by IL-13 Gene Therapy in Rheumatoid Arthritis Synovium. J. Immunol. 165: 2755-2763 [Abstract] [Full Text]  
• Banzai, Y., Miki, H., Yamaguchi, H., Takenawa, T. (2000). Essential Role of Neural Wiskott-Aldrich Syndrome Protein in Neurite Extension in PC12 Cells and Rat Hippocampal Primary Culture Cells. J. Biol. Chem. 275: 11987-11992 [Abstract] [Full Text]  
• Fujii, M., Takeda, K., Imamura, T., Aoki, H., Sampath, T. K., Enomoto, S., Kawabata, M., Kato, M., Ichijo, H., Miyazono, K. (1999). Roles of Bone Morphogenetic Protein Type I Receptors and Smad Proteins in Osteoblast and Chondroblast Differentiation. Mol. Biol. Cell 10: 3801-3813 [Abstract] [Full Text]  
• Ohba, M., Ishino, K., Kashiwagi, M., Kawabe, S., Chida, K., Huh, N.-H., Kuroki, T. (1998). Induction of Differentiation in Normal Human Keratinocytes by Adenovirus-Mediated Introduction of the eta  and delta  Isoforms of Protein Kinase C. Mol. Cell. Biol. 18: 5199-5207 [Abstract] [Full Text]  
• Terada, K., Nakako, T., Yang, X.-L., Iida, M., Aiba, N., Minamiya, Y., Nakai, M., Sakaki, T., Miura, N., Sugiyama, T. (1998). Restoration of Holoceruloplasmin Synthesis in LEC Rat after Infusion of Recombinant Adenovirus Bearing WND cDNA. J. Biol. Chem. 273: 1815-1820 [Abstract] [Full Text]  
• Ohashi, T., Watabe, K., Uehara, K., Sly, W. S., Vogler, C., Eto, Y. (1997). Adenovirus-mediated gene transfer and expression of human beta -glucuronidase gene in the liver, spleen, and central nervous system in mucopolysaccharidosis type VII mice. Proc. Natl. Acad. Sci. USA 94: 1287-1292 [Abstract] [Full Text]  
• Gojo, S., Kitamura, S., Hatano, O., Takakusu, A., Hashimoto, K., Kanegae, Y., Saito, I. (1997). TRANSPLANTATION OF GENETICALLY MARKED CARDIAC MUSCLE CELLS. J. Thorac. Cardiovasc. Surg. 113: 10-18 [Abstract] [Full Text]  
• Ueno, H., Li, J.-J., Tomita, H., Yamamoto, H., Pan, Y., Kanegae, Y., Saito, I., Takeshita, A. (1995). Quantitative Analysis of Repeat Adenovirus-Mediated Gene Transfer Into Injured Canine Femoral Arteries. Arterioscler. Thromb. Vasc. Bio. 15: 2246-2253 [Abstract] [Full Text]