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J Virol. 1985 June; 54(3): 661-664

Intracellular synthesis of human parainfluenza type 3 virus-specified polypeptides.

S L Wechsler, D M Lambert, M S Galinski and M W Pons

ABSTRACT

The intracellular synthesis of human parainfluenza type 3 virus-specified polypeptides was examined by polyacrylamide gel electrophoresis of [35S]methionine-labeled cell extracts under reducing conditions. All of the virion structural proteins were detected in cell extracts, including: L, 180,000 molecular weight (180K); P, 83K; HN, 69K; NP, 66K; F0, 60K; F1, 51K; and M, 38K. P and NP were phosphorylated. HN and F were glycosylated. The kinetics of intracellular viral protein synthesis did not detect any early or late proteins. Pulse-chase experiments failed to detect any precursor-product relationships. No nonstructural proteins were detected.

J Virol. 1985 June; 54(3): 661-664






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Copyright © 1985 by the American Society for Microbiology. All rights reserved.