This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pipas, J M Search for Related Content PubMed PubMed Citation Articles by Pipas, J M

Mutations near the carboxyl terminus of the simian virus 40 large tumor antigen alter viral host range.

ABSTRACT

We report the characterization of three mutants of simian virus 40 with mutations that delete sequences near the 3' end of the gene encoding large tumor antigen (T antigen). Two of these mutants, dl1066 and dl1140, exhibit an altered viral host range. Wild-type simian virus 40 is capable of undergoing a complete productive infection on several types of established African green monkey kidney lines, including BSC40 and CV1P. dl1066 and dl1140 grow on BSC40 cells at 37 degrees C. However, both mutants fail to form plaques on BSC40 cells at 32 degrees C or on CV1P cells at any temperature. These mutants are capable of replicating viral DNA in the nonpermissive cell type, indicating a defect in an activity of T antigen not related to its replication function. Furthermore this defect can be complemented in trans by the wild type or by a variety of DNA replication-negative T antigen mutants, so long as they produce a normal carboxyl-terminal region of the molecule. Our data are consistent with the hypothesis that the C-terminal region of T antigen constitutes a functional domain. We propose that this domain encodes an activity that is required for simian virus 40 productive infection on the CV1P cell line, but not on BSC40.

This article has been cited by other articles:

• Zhao, X., Madden-Fuentes, R. J., Lou, B. X., Pipas, J. M., Gerhardt, J., Rigell, C. J., Fanning, E. (2008). Ataxia Telangiectasia-Mutated Damage-Signaling Kinase- and Proteasome-Dependent Destruction of Mre11-Rad50-Nbs1 Subunits in Simian Virus 40-Infected Primate Cells. J. Virol. 82: 5316-5328 [Abstract] [Full Text]  
• Khopde, S., Simmons, D. T. (2008). Simian Virus 40 DNA Replication Is Dependent on an Interaction between Topoisomerase I and the C-Terminal End of T Antigen. J. Virol. 82: 1136-1145 [Abstract] [Full Text]  
• Sroller, V., Vilchez, R. A., Stewart, A. R., Wong, C., Butel, J. S. (2008). Influence of the Viral Regulatory Region on Tumor Induction by Simian Virus 40 in Hamsters. J. Virol. 82: 871-879 [Abstract] [Full Text]  
• Gomez-Llorente, Y., Fletcher, R. J., Chen, X. S., Carazo, J. M., Martin, C. S. (2005). Polymorphism and Double Hexamer Structure in the Archaeal Minichromosome Maintenance (MCM) Helicase from Methanobacterium thermoautotrophicum. J. Biol. Chem. 280: 40909-40915 [Abstract] [Full Text]  
• Cantalupo, P., Doering, A., Sullivan, C. S., Pal, A., Peden, K. W. C., Lewis, A. M., Pipas, J. M. (2005). Complete Nucleotide Sequence of Polyomavirus SA12. J. Virol. 79: 13094-13104 [Abstract] [Full Text]  
• Gai, D., Li, D., Finkielstein, C. V., Ott, R. D., Taneja, P., Fanning, E., Chen, X. S. (2004). Insights into the Oligomeric States, Conformational Changes, and Helicase Activities of SV40 Large Tumor Antigen. J. Biol. Chem. 279: 38952-38959 [Abstract] [Full Text]  
• Poulin, D. L., Kung, A. L., DeCaprio, J. A. (2004). p53 Targets Simian Virus 40 Large T Antigen for Acetylation by CBP. J. Virol. 78: 8245-8253 [Abstract] [Full Text]  
• Vilchez, R. A., Butel, J. S. (2004). Emergent Human Pathogen Simian Virus 40 and Its Role in Cancer. Clin. Microbiol. Rev. 17: 495-508 [Abstract] [Full Text]  
• Roy, R., Trowbridge, P., Yang, Z., Champoux, J. J., Simmons, D. T. (2003). The Cap Region of Topoisomerase I Binds to Sites near Both Ends of Simian Virus 40 T Antigen. J. Virol. 77: 9809-9816 [Abstract] [Full Text]  
• Simon, M. A., Ilyinskii, P. O., Baskin, G. B., Knight, H. Y., Pauley, D. R., Lackner, A. A. (1999). Association of Simian Virus 40 with a Central Nervous System Lesion Distinct from Progressive Multifocal Leukoencephalopathy in Macaques with AIDS. Am. J. Pathol. 154: 437-446 [Abstract] [Full Text]  
• Butel, J. S., Lednicky, J. A. (1999). Cell and Molecular Biology of Simian Virus 40: Implications for Human Infections and Disease. JNCI J Natl Cancer Inst 91: 119a-134a [Abstract] [Full Text]