This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Stahl, H Articles by Knippers, R Search for Related Content PubMed PubMed Citation Articles by Stahl, H Articles by Knippers, R

A large-tumor-antigen-specific monoclonal antibody inhibits DNA replication of simian virus 40 minichromosomes in an in vitro elongation system.

ABSTRACT

In productively infected cells, a fraction of large-tumor antigen (T antigen) is tightly bound to replicating simian virus 40 (SV40) minichromosomes and does not dissociate at salt concentrations of greater than 1 M NaCl. We present electronmicrograms demonstrating the presence of T antigen on the replicated sections of replicating SV40 minichromosomes. We also show that the fraction of tightly bound T antigen is recognized by antibodies from mouse tumor serum and, more specifically, by a particular T-antigen-specific monoclonal antibody, PAb 1630. A second T-antigen-specific monoclonal antibody, PAb 101, does not react with the T-antigen fraction remaining on replicating SV40 chromatin at high salt concentrations. We used an in vitro replication system which allows, via semiconservative DNA replication, the completion of in vivo-initiated replicative intermediate DNA molecules. We show that monoclonal antibody PAb 1630, but not monoclonal antibody PAb 101, inhibits viral DNA replication. We discuss the possibility that SV40 T antigen may play a role in chain elongation during SV40 chromatin replication.

This article has been cited by other articles:

• Love, T. M., de Jesus, R., Kean, J. A., Sheng, Q., Leger, A., Schaffhausen, B. (2005). Activation of CREB/ATF Sites by Polyomavirus Large T Antigen. J. Virol. 79: 4180-4190 [Abstract] [Full Text]  
• Xu, X., Hamhouyia, F., Thomas, S. D., Burke, T. J., Girvan, A. C., McGregor, W. G., Trent, J. O., Miller, D. M., Bates, P. J. (2001). Inhibition of DNA Replication and Induction of S Phase Cell Cycle Arrest by G-rich Oligonucleotides. J. Biol. Chem. 276: 43221-43230 [Abstract] [Full Text]  
• SenGupta, D. J., Borowiec, J. A. (1992). Strand-Specific Recognition of a Synthetic DNA Replication Fork by the SV40 Large Tumor Antigen. Science 256: 1656-1661 [Abstract]  
• Roberts, J., D'Urso, G (1988). An origin unwinding activity regulates initiation of DNA replication during mammalian cell cycle. Science 241: 1486-1489 [Abstract]  
• Dodson, M, Dean, F., Bullock, P, Echols, H, Hurwitz, J (1987). Unwinding of duplex DNA from the SV40 origin of replication by T antigen. Science 238: 964-967 [Abstract]