This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Persson, R Articles by Everitt, E Search for Related Content PubMed PubMed Citation Articles by Persson, R Articles by Everitt, E

 Previous Article  |  Next Article 

J Virol. 1985 April; 54(1): 92-97

Virus-receptor interaction in the adenovirus system: characterization of the positive cooperative binding of virions on HeLa cells.

ABSTRACT

The established positive cooperativity of adenovirus 2 binding to HeLa cells revealed a strong temperature dependence. The degree of cooperativity, quantified by means of Hill coefficients, progressively increased from 10 degrees C to reach a maximum level, which was maintained between 20 and 37 degrees C. On the other hand, negative cooperativity of virion attachment was apparent at 3.0 degrees C and on glutaraldehyde-stabilized cells. The corresponding monovalent ligand of the system, the fiber antigen, demonstrated only weak-positive cooperativity of the binding at 37.0 degrees C, which was absent at 3.0 degrees C. Dithiothreitol and dansylcadaverine, reagents inhibiting clustering of ligand-receptor complexes in the plasma membrane, markedly reduced the degree of positive cooperative binding at 37.0 degrees C. Evidently, the positive cooperative binding of adenovirus to HeLa cells at 37.0 degrees C is a consequence of both the multivalency of virus attachment proteins, i.e., fibers, on the virion and of the capacity of the receptor sites to migrate in the plane of the plasma membrane, forming local aggregates of virus-receptor site complexes.

This article has been cited by other articles:

• Excoffon, K. J. D. A., Gansemer, N., Traver, G., Zabner, J. (2007). Functional Effects of Coxsackievirus and Adenovirus Receptor Glycosylation on Homophilic Adhesion and Adenoviral Infection. J. Virol. 81: 5573-5578 [Abstract] [Full Text]  
• Excoffon, K. J. D. A., Traver, G. L., Zabner, J. (2005). The Role of the Extracellular Domain in the Biology of the Coxsackievirus and Adenovirus Receptor. Am. J. Respir. Cell Mol. Bio. 32: 498-503 [Abstract] [Full Text]  
• THAISOMBOONSUK, B. K., CLAYSON, E. T., PANTUWATANA, S., VAUGHN, D. W., ENDY, T. P. (2005). CHARACTERIZATION OF DENGUE-2 VIRUS BINDING TO SURFACES OF MAMMALIAN AND INSECT CELLS. Am J Trop Med Hyg 72: 375-383 [Abstract] [Full Text]  
• O'Reilly, L., Roth, M. J. (2003). G541R within the 4070A TM Protein Regulates Fusion in Murine Leukemia Viruses. J. Virol. 77: 12011-12021 [Abstract] [Full Text]  
• Shayakhmetov, D. M., Lieber, A. (2000). Dependence of Adenovirus Infectivity on Length of the Fiber Shaft Domain. J. Virol. 74: 10274-10286 [Abstract] [Full Text]  
• Zabner, J., Chillon, M., Grunst, T., Moninger, T. O., Davidson, B. L., Gregory, R., Armentano, D. (1999). A Chimeric Type 2 Adenovirus Vector with a Type 17 Fiber Enhances Gene Transfer to Human Airway Epithelia. J. Virol. 73: 8689-8695 [Abstract] [Full Text]  
• Miyazawa, N., Leopold, P. L., Hackett, N. R., Ferris, B., Worgall, S., Falck-Pedersen, E., Crystal, R. G. (1999). Fiber Swap between Adenovirus Subgroups B and C Alters Intracellular Trafficking of Adenovirus Gene Transfer Vectors. J. Virol. 73: 6056-6065 [Abstract] [Full Text]  
• Davison, E., Kirby, I., Elliott, T., Santis, G. (1999). The Human HLA-A*0201 Allele, Expressed in Hamster Cells, Is Not a High-Affinity Receptor for Adenovirus Type 5 Fiber. J. Virol. 73: 4513-4517 [Abstract] [Full Text]  
• Walters, R. W., Grunst, T., Bergelson, J. M., Finberg, R. W., Welsh, M. J., Zabner, J. (1999). Basolateral Localization of Fiber Receptors Limits Adenovirus Infection from the Apical Surface of Airway Epithelia. J. Biol. Chem. 274: 10219-10226 [Abstract] [Full Text]  
• Liebermann, H., Mentel, R., Bauer, U., Pring-Akerblom, P., Dolling, R., Modrow, S., Seidel, W. (1998). Receptor Binding Sites and Antigenic Epitopes on the Fiber Knob of Human Adenovirus Serotype 3. J. Virol. 72: 9121-9130 [Abstract] [Full Text]  
• Fasbender, A., Zabner, J., Chillon, M., Moninger, T. O., Puga, A. P., Davidson, B. L., Welsh, M. J. (1997). Complexes of Adenovirus with Polycationic Polymers and Cationic Lipids Increase the Efficiency of Gene Transfer in Vitro and in Vivo. J. Biol. Chem. 272: 6479-6489 [Abstract] [Full Text]  
• Walters, R. W., Yi, S. M. P., Keshavjee, S., Brown, K. E., Welsh, M. J., Chiorini, J. A., Zabner, J. (2001). Binding of Adeno-associated Virus Type 5 to 2,3-Linked Sialic Acid Is Required for Gene Transfer. J. Biol. Chem. 276: 20610-20616 [Abstract] [Full Text]