This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Preston, C M Articles by Stow, N D Search for Related Content PubMed PubMed Citation Articles by Preston, C M Articles by Stow, N D

 Previous Article  |  Next Article 

J Virol. 1984 June; 50(3): 708-716

Analysis of DNA sequences which regulate the transcription of a herpes simplex virus immediate early gene.

ABSTRACT

The locations and functions of DNA sequences involved in transcription of the gene encoding herpes simplex virus type 1 immediate early (IE) mRNAs 4 and 5 were analyzed by use of a transient-expression assay. The region upstream of the genes encoding IE mRNAs 4 and 5 was fused to the thymidine kinase gene coding sequences, and production of enzyme or RNA was measured after transfection of plasmids into BHK cells. The effect of deletions in the upstream region was determined in the absence or presence of a virus structural component which stimulates herpes simplex virus IE transcription. Two distinct units were identified. One of these was a promoter which required not more than 69 base pairs of DNA specific for the genes encoding IE mRNAs 4 and 5 upstream from the mRNA 5' terminus. The other was a far-upstream region which mediated the response to the virion component and had an upstream boundary between nucleotides -347 and -335. An origin of DNA replication was interposed between these two units. The element TAATGAGATAC , which represents a consensus sequence present in the upstream regions of all herpes simplex type 1 IE genes, appeared to be essential for stimulation by the virion component. The activity of this element was modulated by the sequences which flank it, especially by regions having extremely high contents of guanine plus cytosine and which contain a conserved unit CCCGCCC or its complement GGGCGGG .

This article has been cited by other articles:

• Lu, R., Misra, V. (2000). Potential Role for Luman, the Cellular Homologue of Herpes Simplex Virus VP16 (alpha Gene trans-Inducing Factor), in Herpesvirus Latency. J. Virol. 74: 934-943 [Abstract] [Full Text]  
• Hughes, T. A., Boissiere, S. L., O'Hare, P. (1999). Analysis of Functional Domains of the Host Cell Factor Involved in VP16 Complex Formation. J. Biol. Chem. 274: 16437-16443 [Abstract] [Full Text]  
• Nguyen-Huynh, A. T., Schaffer, P. A. (1998). Cellular Transcription Factors Enhance Herpes Simplex Virus Type 1 oriS-Dependent DNA Replication. J. Virol. 72: 3635-3645 [Abstract] [Full Text]  
• Tanaka, M, Grossniklaus, U, Herr, W, Hernandez, N (1988). Activation of the U2 snRNA promoter by the octamer motif defines a new class of RNA polymerase II enhancer elements.. Genes Dev. 2: 1764-1778 [Abstract]  
• Baumruker, T, Sturm, R, Herr, W (1988). OBP100 binds remarkably degenerate octamer motifs through specific interactions with flanking sequences.. Genes Dev. 2: 1400-1413 [Abstract]  
• Triezenberg, S J, LaMarco, K L, McKnight, S L (1988). Evidence of DNA: protein interactions that mediate HSV-1 immediate early gene activation by VP16.. Genes Dev. 2: 730-742 [Abstract]  
• Homa, F L, Glorioso, J C, Levine, M (1988). A specific 15-bp TATA box promoter element is required for expression of a herpes simplex virus type 1 late gene.. Genes Dev. 2: 40-53 [Abstract]