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J Virol. 1970 June; 5(6): 795-800
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Stalked Bacteria: Properties of Deoxriybonucleic Acid Bacteriophage CbK

Department of Molecular Biology, Division of Biological Sciences, Albert Einstein College of Medicine, Bronx, New York 10461

ABSTRACT

The Caulobacter crescentus bacteriophage CbK was studied with respect to the physical and chemical properties of both the phage and its deoxyribonucleic acid (DNA). Electron micrographs reveal the phage to be among the largest DNA bacteriophages reported, with head dimensions of 64 by 195 nm and a flexible tail 275 nm in length. The phage is composed of 57% DNA. This DNA is double-stranded as indicated by (i) the sharp increase in extinction coefficient over a narrow range of temperature increase, (ii) an increase in density in CsCl upon thermal denaturation, and (iii) the equivalence of guanine and cytosine as well as adenine and thymine, determined by chemical analysis. CbK DNA cosediments with Escherichia coli phage T2 DNA and has therefore been assigned an S_20,w value of 63.5S. The size of the phage and its DNA and the percentage of DNA indicate that the CbK phage head is relatively loosely packed. The properties of the DNA from bacteriophage CbK are similar to those of host C. crescentus DNA with respect to buoyant density, thermal transition point, and guanine plus cytosine content.

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