This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Berns, K. I. Articles by Rose, J. A. Search for Related Content PubMed PubMed Citation Articles by Berns, K. I. Articles by Rose, J. A.

Evidence for a Single-Stranded Adenovirus-Associated Virus Genome: Isolation and Separation of Complementary Single Strands

Laboratory of Biology of Viruses, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20014

ABSTRACT

Single-stranded adenovirus-associated virus type 2 deoxyribonucleic acid (AAV-2 DNA) has been isolated from the virion after enzymatic pretreatment of the particles by heating at 53 C for 1 hr in 0.015 M NaCl plus 0.0015 M sodium citrate in the presence of 1% sodium dodecyl sulfate. Double-stranded AAV-2 DNA present as a marker is not denatured by this treatment. AAV-2 single-stranded DNA is composed of two complementary species which can be separated in neutral CsCl when 5-bromodeoxyuridine has been substituted for thymidine in the DNA. The present report is the first documented instance of the separation of complementary strands of an animal virus DNA.

This article has been cited by other articles:

• Ding, C., Urabe, M., Bergoin, M., Kotin, R. M. (2002). Biochemical Characterization of Junonia coenia Densovirus Nonstructural Protein NS-1. J. Virol. 76: 338-345 [Abstract] [Full Text]  
• Trahair, T. N., Alexander, I. E., Rowe, P. B., Smythe, J. A. (2000). The adenovirus E4 ORF6 and E1b 55 kDa proteins cooperate in a p53-independent manner to enhance transduction by recombinant adeno-associated virus vectors. J. Gen. Virol. 81: 2983-2991 [Abstract] [Full Text]  
• Nakai, H., Storm, T. A., Kay, M. A. (2000). Recruitment of Single-Stranded Recombinant Adeno-Associated Virus Vector Genomes and Intermolecular Recombination Are Responsible for Stable Transduction of Liver In Vivo. J. Virol. 74: 9451-9463 [Abstract] [Full Text]  
• Malik, A. K., Monahan, P. E., Allen, D. L., Chen, B.-G., Samulski, R. J., Kurachi, K. (2000). Kinetics of Recombinant Adeno-Associated Virus-Mediated Gene Transfer. J. Virol. 74: 3555-3565 [Abstract] [Full Text]  
• Vincent-Lacaze, N., Snyder, R. O., Gluzman, R., Bohl, D., Lagarde, C., Danos, O. (1999). Structure of Adeno-Associated Virus Vector DNA following Transduction of the Skeletal Muscle. J. Virol. 73: 1949-1955 [Abstract] [Full Text]  
• Summerford, C., Samulski, R. J. (1998). Membrane-Associated Heparan Sulfate Proteoglycan Is a Receptor for Adeno-Associated Virus Type 2 Virions. J. Virol. 72: 1438-1445 [Abstract] [Full Text]