JVI Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, R.
Right arrow Articles by Green, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, R.
Right arrow Articles by Green, H.

 Previous Article  |  Next Article 

J Virol. 1970 June; 5(6): 677-681
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Human-Mouse Hybrid Cell Lines and Susceptibility to Poliovirus 1

I. Conversion from Polio Sensitivity to Polio Resistance Accompanying Loss of Human Gene-Dependent Polio Receptors

Richard Wang2, Robert Pollack, Toshihisa Kusano3 and Howard Green

Departments of Cell Biology and Pathology, New York University School of Medicine, New York, New York 10016

ABSTRACT

A number of human-mouse somatic hybrid cell lines have been prepared, containing from 3 to 12 human biarmed chromosomes. These lines were susceptible to poliovirus type 1, producing viral yields comparable to those of the human parental cells. A small proportion of the cells of these lines survived the polio infection, and their progeny were solidly resistant to reinfection with the virus. Both sensitive and resistant hybrids produced virus following infection with viral ribonucleic acid, indicating that the cytoplasm of the resistant hybrids was able to support viral multiplication. Viral adsorption studies carried out at 4 C showed that the resistant sublines had negligible ability to adsorb the virus. It was concluded that the hybrid cells became resistant to polio through loss of the human chromosome bearing the gene for the receptor substance.

FOOTNOTES

2 Fellow of the National Cancer Institute (5F02 CA40936).

3 Present address: Research Institute for Tuberculosis, Leprosy, and Cancer, Tohoku University, Sendai, Japan.

1 Aided by grants from the National Cancer Institute.

J Virol. 1970 June; 5(6): 677-681
Copyright © 1970 American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Mol. Cell. Biol. Microbiol. Mol. Biol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 1970 by the American Society for Microbiology. All rights reserved.