This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Tan, K. B. Search for Related Content PubMed PubMed Citation Articles by Tan, K. B.

Electron Microscopy of Cells Infected with Semliki Forest Virus Temperature-Sensitive Mutants: Correlation of Ultrastructural and Physiological Observations

Department of Microbiology, John Curtin School of Medical Research, Australian National University, Canberra, Australia

ABSTRACT

Cells infected at the permissive temperature with three temperature-sensitive mutants of Semliki Forest virus were not significantly different in appearance from cells infected, at either the permissive or nonpermissive temperature, with wild-type virus. Virus particles, nucleocapsids, spherules, and tubules were seen in the cytoplasm. But replication of the mutants was inhibited in cells infected at the nonpermissive temperature. This was evidenced by the absence of virus particles and nucleocapsids (except in one case) and the absence or limited production of spherules and tubules. These observations are discussed with reference to the physiological defects of the mutants.

¹ Present address: Department of Microbiology, Roche Institute of Molecular Biology, Nutley, N.J. 07110.