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J Virol. 1970 May; 5(5): 624-631
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Carrier Cultures of Simian Foamy Virus

J. K. Clarke1, Janis Samuels, Evelyn Dermott and F. W. Gay

Department of Microbiology, The Queen's University of Belfast, Belfast BT12 6BN, Northern Ireland

ABSTRACT

The production of cultures of HEp-2 and BHK-21 cells persistently infected with a type 1 simian foamy virus is described. After infection, HEp-2 cells showed no structural changes, whereas BHK-21 cells lost their normal spindle shape and showed mitochondrial damage, and some cells contained many lysosomes. Thin sections also showed that a few BHK-21 cells contained virus particles in low concentration, and infectious virus could be isolated from both the cells and the supernatant fluid. No virus was seen in thin sections of HEp-2 cells, although infectious virus in low titer could be recovered intermittently from lysed cells. Both carrier cultures were immune to challenge with homologous virus and antigen could be detected in over 90% of the cells even after growth for 9 weeks in the presence of virus-neutralizing serum. The distribution of antigen in carrier cultures of both cell types is described and compared with that seen in cytocidal infections.

FOOTNOTES

1 Present address: Veterinary Research Laboratories, Stormont, Belfast, BT4 3SD, Northern Ireland.

J Virol. 1970 May; 5(5): 624-631
Copyright © 1970 American Society for Microbiology. All Rights Reserved.



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