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J Virol. 1970 March; 5(3): 309-312
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Uncoating of Poliovirus by Isolated Plasma Membranes

Department of Microbiology, Yale University School of Medicine, New Haven, Connecticut 06510

ABSTRACT

Poliovirus type I LSc strain labeled with ¹⁴C-uridine was adsorbed onto isolated plasma membranes and incubated with them. When membranes from Hep-2 or Vero cells were used, 22% of the label was converted to a trichloroacetic acid-soluble form, when trypsin or ribonuclease was added, the fraction rendered soluble was increased, and when the two enzymes were added in sequence, 85% or more of the label became trichloroacetic acid-soluble. This labilization of poliovirus could be reproduced when butanol-solubilized proteins from membranes were substituted for the whole plasma membranes, but it did not occur with membranes from polio-virus-resistant calf kidney or BHK-21 cells.

¹ Present address: Institute of Hygiene, University of the Philippines, Ermita, Manila, Philippines.