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Lack of Nucleotide Sequence Relationship Among the Temperate Bacteriophage SPO2, Bacillus subtilis, and Virulent Bacteriophages ß3 and ß22

Division of Biochemistry, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D.C. 20012

ABSTRACT

Radiolabeled phage SPO2 fragments were tested for the abiity to form interspecies deoxyribonucleic acid (DNA) duplexes with DNA from Bacillus subtilis and from phages ß3 and ß22. No reassociation, above control values, occurred between the DNA of phage SPO2 and that of its host or either of the virulent B. subtilis phages. It appears that extensive nucleotide sequence similarities between portions of host and phage DNA species are not necessary for the formation of the lysogenic state. The thermal elution profile of reassociated SPO2 DNA exhibited a normal distribution, indicating no heterogeneity in base composition. This differs from temperate enterophages whose DNA molecules contain regions of differing base composition.

¹ Present address: Department of Genetics, Stanford University School of Medicine, Stanford, Calif. 94305