This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Deeb, S. S. Search for Related Content PubMed PubMed Citation Articles by Deeb, S. S.

 Previous Article  |  Next Article 

J Virol. 1970 January; 5(1): 27-31
Copyright © 1970 American Society for Microbiology. All Rights Reserved.

Studies on the In Vitro Assembly of Bacteriophage 80 and 80-Lambda Hybrids

Biology Department, American University at Bierut, Beirut, Lebanon

ABSTRACT

Suppressor-sensitive (sus) mutants of bacteriophage 80 defective in late functions were classified, by means of in vitro assembly tests, into two complementation groups: head donors and tail donors. Each group of mutants was subdivided, by means of two-factor crosses, into six cistrons. Deletion mapping revealed clustering of tail and also of head cistrons. The two clusters were located in the left arm of vegetative 80 (the tail specifying cluster being distal). In vitro cross complementation between 80 and lambda sus mutants revealed that whereas lambda heads could quite efficiently bind 80 tails to form viable phage, the union of 80 heads and lambda tails was very much less efficient. Deletion mapping of the 80 sus mutants, using both 80 and i⁸⁰h deletion lysogens indicated congruent gross gene arrangement in the two related bacteriophages.