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Characterization of Bluetongue Virus Ribonucleic Acid

Molecular Biology Section, Veterinary Research Institute, Onderstepoort, South Africa

ABSTRACT

An improved purification procedure yielded bluetongue virus free from any single-stranded ribonucleic acid (RNA) component. Double-stranded RNA obtained from purified virus or isolated from infected cells was fractionated into 5 components by means of sucrose gradient sedimentation analysis, and into 10 components by electrophoresis on polyacrylamide gels. The size of these components vary from 0.5 x 10⁶ to 2.8 x 10⁶ daltons, with a total molecular weight estimate of about 1.5 x 10⁷ for the viral nucleic acid. The denaturation of the genome and separation of the resulting fragments are also discussed.

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