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J Virol. 1983 November; 48(2): 352-360

Isolation of monoclonal antibodies that recognize the transforming proteins of avian sarcoma viruses.

ABSTRACT

Thirteen clones of hybrid cells which synthesize antibodies directed against the Rous sarcoma virus (RSV) transforming protein, pp60src, were isolated. Mouse myeloma cells were fused with spleen cells from mice that had been immunized with purified pp60src from bacterial recombinants which direct the synthesis of the RSV src gene. The hybridomas which survived the selection medium were screened by immunoprecipitation of pp60src from 32P-labeled lysates of RSV-transformed cells. Monoclonal antibodies produced by subclones derived from 13 hybridomas recognized pp60src encoded by the Schmidt-Ruppin and Prague strains of RSV and the cellular homolog of pp60src. Antibody from clone 261 had a high affinity for the viral yes gene product, and antibodies from clones 443 and 463 recognized the transforming proteins encoded by viruses containing the related transforming genes fps and ros. Several other clones had a low affinity for the viral yes, fps, and ros gene products which could be detected by in vitro phosphorylation of the transforming proteins after immunoprecipitation with the monoclonal antibody. All of the monoclonal antibodies allowed phosphorylation of pp60src and casein in an immune complex-bound reaction.

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• Iwasaki, T., Sato, K.-i., Yoshino, K.-i., Itakura, S., Kosuge, K., Tokmakov, A. A., Owada, K., Yonezawa, K., Fukami, Y. (2006). Phylogeny of Vertebrate Src Tyrosine Kinases Revealed by the Epitope Region of mAb327.. J Biochem 139: 347-354 [Abstract] [Full Text]  
• Neron, S., Suck, G., Ma, X.-Z., Sakac, D., Roy, A., Katsman, Y., Dussault, N., Racine, C., Branch, D. R. (2006). B cell proliferation following CD40 stimulation results in the expression and activation of Src protein tyrosine kinase. Int Immunol 18: 375-387 [Abstract] [Full Text]  
• Gomes-Solecki, M. J. C., Savitt, A. G., Rowehl, R., Glass, J. D., Bliska, J. B., Dattwyler, R. J. (2005). LcrV Capture Enzyme-Linked Immunosorbent Assay for Detection of Yersinia pestis from Human Samples. CVI 12: 339-346 [Abstract] [Full Text]  
• Nixon, R. R. (2005). Prion-associated Increases in Src-family Kinases. J. Biol. Chem. 280: 2455-2462 [Abstract] [Full Text]  
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• Wrobel, C. N., Debnath, J., Lin, E., Beausoleil, S., Roussel, M. F., Brugge, J. S. (2004). Autocrine CSF-1R activation promotes Src-dependent disruption of mammary epithelial architecture. J. Cell Biol. 165: 263-273 [Abstract] [Full Text]  
• Seabold, G. K., Burette, A., Lim, I. A., Weinberg, R. J., Hell, J. W. (2003). Interaction of the Tyrosine Kinase Pyk2 with the N-Methyl-D-aspartate Receptor Complex via the Src Homology 3 Domains of PSD-95 and SAP102. J. Biol. Chem. 278: 15040-15048 [Abstract] [Full Text]  
• Wang, Y., Mishra, R., Simonson, M. S. (2003). Ca2+/Calmodulin-Dependent Protein Kinase II Stimulates c-fos Transcription and DNA Synthesis by a Src-Based Mechanism in Glomerular Mesangial Cells. J. Am. Soc. Nephrol. 14: 28-36 [Abstract] [Full Text]  
• Liu, H.-Y., Meakin, S. O. (2002). ShcB and ShcC Activation by the Trk Family of Receptor Tyrosine Kinases. J. Biol. Chem. 277: 26046-26056 [Abstract] [Full Text]  
• Kamei, T., Jones, S. R., Chapman, B. M., MCGonigle, K. L., Dai, G., Soares, M. J. (2002). The Phosphatidylinositol 3-Kinase/Akt Signaling Pathway Modulates the Endocrine Differentiation of Trophoblast Cells. Mol. Endocrinol. 16: 1469-1481 [Abstract] [Full Text]  
• Obergfell, A., Eto, K., Mocsai, A., Buensuceso, C., Moores, S. L., Brugge, J. S., Lowell, C. A., Shattil, S. J. (2002). Coordinate interactions of Csk, Src, and Syk kinases with {alpha}IIb{beta}3 initiate integrin signaling to the cytoskeleton. J. Cell Biol. 157: 265-275 [Abstract] [Full Text]  
• Kato, M., Takeda, K., Kawamoto, Y., Iwashita, T., Akhand, A. A., Senga, T., Yamamoto, M., Sobue, G., Hamaguchi, M., Takahashi, M., Nakashima, I. (2002). Repair by Src Kinase of Function-impaired RET with Multiple Endocrine Neoplasia Type 2A Mutation with Substitutions of Tyrosines in the COOH-Terminal Kinase Domain for Phenylalanine. Cancer Res. 62: 2414-2422 [Abstract] [Full Text]  
• Selbach, M., Moese, S., Hauck, C. R., Meyer, T. F., Backert, S. (2002). Src Is the Kinase of the Helicobacter pylori CagA Protein in Vitro and in Vivo. J. Biol. Chem. 277: 6775-6778 [Abstract] [Full Text]  
• Sohara, Y., Ishiguro, N., Machida, K., Kurata, H., Thant, A. A., Senga, T., Matsuda, S., Kimata, K., Iwata, H., Hamaguchi, M. (2001). Hyaluronan Activates Cell Motility of v-Src-transformed Cells via Ras-Mitogen-activated Protein Kinase and Phosphoinositide 3-Kinase-Akt in a Tumor-specific Manner. Mol. Biol. Cell 12: 1859-1868 [Abstract] [Full Text]  
• Bijlmakers, M.-J. J. E., Marsh, M. (2000). Hsp90 Is Essential for the Synthesis and Subsequent Membrane Association, But Not the Maintenance, of the Src-Kinase p56lck. Mol. Biol. Cell 11: 1585-1595 [Abstract] [Full Text]  
• Akhand, A. A., Pu, M., Senga, T., Kato, M., Suzuki, H., Miyata, T., Hamaguchi, M., Nakashima, I. (1999). Nitric Oxide Controls Src Kinase Activity through a Sulfhydryl Group Modification-mediated Tyr-527-independent and Tyr-416-linked Mechanism. J. Biol. Chem. 274: 25821-25826 [Abstract] [Full Text]  
• Ogris, E., Mudrak, I., Mak, E., Gibson, D., Pallas, D. C. (1999). Catalytically Inactive Protein Phosphatase 2A Can Bind to Polyomavirus Middle Tumor Antigen and Support Complex Formation with pp60c-src. J. Virol. 73: 7390-7398 [Abstract] [Full Text]  
• Owen, J. D., Ruest, P. J., Fry, D. W., Hanks, S. K. (1999). Induced Focal Adhesion Kinase (FAK) Expression in FAK-Null Cells Enhances Cell Spreading and Migration Requiring Both Auto- and Activation Loop Phosphorylation Sites and Inhibits Adhesion-Dependent Tyrosine Phosphorylation of Pyk2. Mol. Cell. Biol. 19: 4806-4818 [Abstract] [Full Text]  
• Arbet-Engels, C., Tartare-Deckert, S., Eckhart, W. (1999). C-terminal Src Kinase Associates with Ligand-stimulated Insulin-like Growth Factor-I Receptor. J. Biol. Chem. 274: 5422-5428 [Abstract] [Full Text]  
• Merkel, K. D., Erdmann, J. M., McHugh, K. P., Abu-Amer, Y., Ross, F. P., Teitelbaum, S. L. (1999). Tumor Necrosis Factor-{alpha} Mediates Orthopedic Implant Osteolysis. Am. J. Pathol. 154: 203-210 [Abstract] [Full Text]  
• Brown, M. T., Andrade, J., Radhakrishna, H., Donaldson, J. G., Cooper, J. A., Randazzo, P. A. (1998). ASAP1, a Phospholipid-Dependent Arf GTPase-Activating Protein That Associates with and Is Phosphorylated by Src. Mol. Cell. Biol. 18: 7038-7051 [Abstract] [Full Text]  
• Abu-Amer, Y., Ross, F. P., McHugh, K. P., Livolsi, A., Peyron, J.-F., Teitelbaum, S. L. (1998). Tumor Necrosis Factor-alpha Activation of Nuclear Transcription Factor-kappa B in Marrow Macrophages Is Mediated by c-Src Tyrosine Phosphorylation of Ikappa Balpha. J. Biol. Chem. 273: 29417-29423 [Abstract] [Full Text]  
• Chang, B. Y., Conroy, K. B., Machleder, E. M., Cartwright, C. A. (1998). RACK1, a Receptor for Activated C Kinase and a Homolog of the beta  Subunit of G Proteins, Inhibits Activity of Src Tyrosine Kinases and Growth of NIH 3T3 Cells. Mol. Cell. Biol. 18: 3245-3256 [Abstract] [Full Text]  
• DeMali, K. A., Kazlauskas, A. (1998). Activation of Src Family Members Is Not Required for the Platelet-Derived Growth Factor beta  Receptor To Initiate Mitogenesis. Mol. Cell. Biol. 18: 2014-2022 [Abstract] [Full Text]  
• Gelderloos, J. A., Rosenkranz, S., Bazenet, C., Kazlauskas, A. (1998). A Role for Src in Signal Relay by the Platelet-derived Growth Factor alpha  Receptor. J. Biol. Chem. 273: 5908-5915 [Abstract] [Full Text]  
• Schwartzberg, P. L., Xing, L., Hoffmann, O., Lowell, C. A., Garrett, L., Boyce, B. F., Varmus, H. E. (1997). Rescue of osteoclast function by transgenic expression of kinase-deficient Src in src-/- mutant mice. Genes Dev. 11: 2835-2844 [Abstract] [Full Text]  
• Somani, A.-K., Bignon, J. S., Mills, G. B., Siminovitch, K. A., Branch, D. R. (1997). Src Kinase Activity Is Regulated by the SHP-1 Protein-tyrosine Phosphatase. J. Biol. Chem. 272: 21113-21119 [Abstract] [Full Text]  
• Abu-Amer, Y., Ross, F. P., Schlesinger, P., Tondravi, M. M., Teitelbaum, S. L. (1997). Substrate Recognition by Osteoclast Precursors Induces C-src/Microtubule Association. J. Cell Biol. 137: 247-258 [Abstract] [Full Text]  
• Beggs, H. E., Baragona, S. C., Hemperly, J. J., Maness, P. F. (1997). NCAM140 Interacts with the Focal Adhesion Kinase p125fak and the SRC-related Tyrosine Kinase p59fyn. J. Biol. Chem. 272: 8310-8319 [Abstract] [Full Text]  
• Davidson, D., Chow, L. M.L., Veillette, A. (1997). Chk, a Csk Family Tyrosine Protein Kinase, Exhibits Csk-like Activity in Fibroblasts, but Not in an Antigen-specific T-cell Line. J. Biol. Chem. 272: 1355-1362 [Abstract] [Full Text]  
• Uetz, P., Fumagalli, S., James, D., Zeller, R. (1996). Molecular Interaction between Limb Deformity Proteins (Formins) and Src Family Kinases. J. Biol. Chem. 271: 33525-33530 [Abstract] [Full Text]  
• Peterson, J. E., Kulik, G., Jelinek, T., Reuter, C. W.M., Shannon, J. A., Weber, M. J. (1996). Src Phosphorylates the Insulin-like Growth Factor Type I Receptor on the Autophosphorylation Sites. REQUIREMENT FOR TRANSFORMATION BY src. J. Biol. Chem. 271: 31562-31571 [Abstract] [Full Text]  
• Luttrell, L. M., Hawes, B. E., van Biesen, T., Luttrell, D. K., Lansing, T. J., Lefkowitz, R. J. (1996). Role of c-Src Tyrosine Kinase in G Protein-coupled Receptorand Gbeta gamma Subunit-mediated Activation of Mitogen-activated Protein Kinases. J. Biol. Chem. 271: 19443-19450 [Abstract] [Full Text]  
• Broome, M. A., Hunter, T. (1996). Requirement for c-Src Catalytic Activity and the SH3 Domain in Platelet-derived Growth Factor BB and Epidermal Growth Factor Mitogenic Signaling. J. Biol. Chem. 271: 16798-16806 [Abstract] [Full Text]  
• Nakamoto, T., Sakai, R., Ozawa, K., Yazaki, Y., Hirai, H. (1996). Direct Binding of C-terminal Region of p130[IMAGE] to SH2 and SH3 Domains of Src Kinase. J. Biol. Chem. 271: 8959-8965 [Abstract] [Full Text]  
• Kawakatsu, H., Sakai, T., Takagaki, Y., Shinoda, Y., Saito, M., Owada, M. K., Yano, J. (1996). A New Monoclonal Antibody Which Selectively Recognizes the Active Form of Src Tyrosine Kinase. J. Biol. Chem. 271: 5680-5685 [Abstract] [Full Text]  
• Osusky, M., Taylor, S. J., Shalloway, D. (1995). Autophosphorylation of Purified c-Src at its Primary Negative Regulation Site. J. Biol. Chem. 270: 25729-25732 [Abstract] [Full Text]  
• Bjorge, J. D., Bellagamba, C., Cheng, H.-C., Tanaka, A., Wang, J. H., Fujita, D. J. (1995). Characterization of Two Activated Mutants of Human pp60[IMAGE][IMAGE] That Escape c-Src Kinase Regulation by Distinct Mechanisms. J. Biol. Chem. 270: 24222-24228 [Abstract] [Full Text]  
• Calautti, E, Missero, C, Stein, P L, Ezzell, R M, Dotto, G P (1995). fyn tyrosine kinase is involved in keratinocyte differentiation control.. Genes Dev. 9: 2279-2291 [Abstract]  
• Yu, C., Meyer, D., Campbell, G., Larner, A., Carter-Su, C, Schwartz, J, Jove, R (1995). Enhanced DNA-binding activity of a Stat3-related protein in cells transformed by the Src oncoprotein. Science 269: 81-83 [Abstract]  
• Loo, L. W. M., Berestecky, J. M., Kanemitsu, M. Y., Lau, A. F. (1995). pp60[IMAGE] -mediated Phosphorylation of Connexin 43, a Gap Junction Protein. J. Biol. Chem. 270: 12751-12761 [Abstract] [Full Text]  
• Valgeirsdóttir, S., Eriksson, A., Nistér, M., Heldin, C.-H., Westermark, B., Claesson-Welsh, L. (1995). Compartmentalization of Autocrine Signal Transduction Pathways in Sis-transformed NIH 3T3 Cells. J. Biol. Chem. 270: 10161-10170 [Abstract] [Full Text]  
• Ariyoshi, H., Oda, A., Salzman, E. W. (1995). Participation of Calpain in Protein-Tyrosine Phosphorylation and Dephosphorylation in Human Blood Platelets. Arterioscler. Thromb. Vasc. Bio. 15: 511-514 [Abstract] [Full Text]  
• Lowell, C A, Soriano, P, Varmus, H E (1994). Functional overlap in the src gene family: inactivation of hck and fgr impairs natural immunity.. Genes Dev. 8: 387-398 [Abstract]  
• David-Pfeuty, T, Bagrodia, S, Shalloway, D (1993). Differential localization patterns of myristoylated and nonmyristoylated c-Src proteins in interphase and mitotic c-Src overexpresser cells. J. Cell Sci. 105: 613-628 [Abstract]  
• Boschelli, F, Uptain, S., Lightbody, J. (1993). The lethality of p60v-src in Saccharomyces cerevisiae and the activation of p34CDC28 kinase are dependent on the integrity of the SH2 domain. J. Cell Sci. 105: 519-528 [Abstract]  
• Thomas, J., Soriano, P, Brugge, J. (1991). Phosphorylation of c-Src on tyrosine 527 by another protein tyrosine kinase. Science 254: 568-571 [Abstract]  
• Matsuda, M, Mayer, B., Fukui, Y, Hanafusa, H (1990). Binding of transforming protein, P47gag-crk, to a broad range of phosphotyrosine-containing proteins. Science 248: 1537-1539 [Abstract]  
• Azarnia, R, Reddy, S, Kmiecik, T., Shalloway, D, Loewenstein, W. (1988). The cellular src gene product regulates junctional cell-to-cell communication. Science 239: 398-401 [Abstract]  
• Martinez, R, Mathey-Prevot, B, Bernards, A, Baltimore, D (1987). Neuronal pp60c-src contains a six-amino acid insertion relative to its non-neuronal counterpart. Science 237: 411-415 [Abstract]  
• Brugge, J, Cotton, P, Lustig, A, Yonemoto, W, Lipsich, L, Coussens, P, Barrett, J N, Nonner, D, Keane, R W (1987). Characterization of the altered form of the c-src gene product in neuronal cells.. Genes Dev. 1: 287-296 [Abstract]  
• Lynch, S., Brugge, J., Levine, J. (1986). Induction of altered c-src product during neural differentiation of embryonal carcinoma cells. Science 234: 873-876 [Abstract]  
• Cooper, J., Gould, K., Cartwright, C., Hunter, T (1986). Tyr527 is phosphorylated in pp60c-src: implications for regulation. Science 231: 1431-1434 [Abstract]  
• Chang, B. Y., Chiang, M., Cartwright, C. A. (2001). The Interaction of Src and RACK1 Is Enhanced by Activation of Protein Kinase C and Tyrosine Phosphorylation of RACK1. J. Biol. Chem. 276: 20346-20356 [Abstract] [Full Text]  
• Sanna, P. P., Berton, F., Cammalleri, M., Tallent, M. K., Siggins, G. R., Bloom, F. E., Francesconi, W. (2000). A role for Src kinase in spontaneous epileptiform activity in the CA3 region of the hippocampus. Proc. Natl. Acad. Sci. USA 97: 8653-8657 [Abstract] [Full Text]  
• Obergfell, A., Eto, K., Mocsai, A., Buensuceso, C., Moores, S. L., Brugge, J. S., Lowell, C. A., Shattil, S. J. (2002). Coordinate interactions of Csk, Src, and Syk kinases with {alpha}IIb{beta}3 initiate integrin signaling to the cytoskeleton. J. Cell Biol. 157: 265-275 [Abstract] [Full Text]