This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Merchlinsky, M J Articles by Ward, D C Search for Related Content PubMed PubMed Citation Articles by Merchlinsky, M J Articles by Ward, D C

 Previous Article  |  Next Article 

J Virol. 1983 July; 47(1): 227-232

Construction of an infectious molecular clone of the autonomous parvovirus minute virus of mice.

ABSTRACT

The linear single-stranded DNA genome of minute virus of mice, an autonomous parvovirus, was cloned in duplex form into the bacterial plasmid pBR322. The recombinant clones of minute virus of mice were infectious when transfected into monolayers of human 324K cells and produced virus plaques with an efficiency of about 6% that obtained with duplex replicative-form DNA purified from cells infected with minute virus of mice. Southern blot analysis of transfected cells indicated that the cloned minute virus of mice genome requires both termini to be intact for excision and replication as a linear duplex molecule.

This article has been cited by other articles:

• Lopez-Bueno, A., Segovia, J. C., Bueren, J. A., O'Sullivan, M. G., Wang, F., Tattersall, P., Almendral, J. M. (2008). Evolution to Pathogenicity of the Parvovirus Minute Virus of Mice in Immunodeficient Mice Involves Genetic Heterogeneity at the Capsid Domain That Determines Tropism. J. Virol. 82: 1195-1203 [Abstract] [Full Text]  
• Lopez-Bueno, A., Rubio, M.-P., Bryant, N., McKenna, R., Agbandje-McKenna, M., Almendral, J. M. (2006). Host-Selected Amino Acid Changes at the Sialic Acid Binding Pocket of the Parvovirus Capsid Modulate Cell Binding Affinity and Determine Virulence. J. Virol. 80: 1563-1573 [Abstract] [Full Text]  
• Choi, E.-Y., Newman, A. E., Burger, L., Pintel, D. (2005). Replication of Minute Virus of Mice DNA Is Critically Dependent on Accumulated Levels of NS2. J. Virol. 79: 12375-12381 [Abstract] [Full Text]  
• Rubio, M.-P., Lopez-Bueno, A., Almendral, J. M. (2005). Virulent Variants Emerging in Mice Infected with the Apathogenic Prototype Strain of the Parvovirus Minute Virus of Mice Exhibit a Capsid with Low Avidity for a Primary Receptor. J. Virol. 79: 11280-11290 [Abstract] [Full Text]  
• Hendrie, P. C., Hirata, R. K., Russell, D. W. (2003). Chromosomal Integration and Homologous Gene Targeting by Replication-Incompetent Vectors Based on the Autonomous Parvovirus Minute Virus of Mice. J. Virol. 77: 13136-13145 [Abstract] [Full Text]  
• Miller, C. L., Pintel, D. J. (2002). Interaction between Parvovirus NS2 Protein and Nuclear Export Factor Crm1 Is Important for Viral Egress from the Nucleus of Murine Cells. J. Virol. 76: 3257-3266 [Abstract] [Full Text]  
• Söderlund-Venermo, M., Riley, L. K., Pintel, D. J. (2001). Construction and initial characterization of an infectious plasmid clone of a newly identified hamster parvovirus. J. Gen. Virol. 82: 919-927 [Abstract] [Full Text]  
• Clément, N., Avalosse, B., El Bakkouri, K., Velu, T., Brandenburger, A. (2001). Cloning and Sequencing of Defective Particles Derived from the Autonomous Parvovirus Minute Virus of Mice for the Construction of Vectors with Minimal cis-Acting Sequences. J. Virol. 75: 1284-1293 [Abstract] [Full Text]  
• Maroto, B., Ramírez, J. C., Almendral, J. M. (2000). Phosphorylation Status of the Parvovirus Minute Virus of Mice Particle: Mapping and Biological Relevance of the Major Phosphorylation Sites. J. Virol. 74: 10892-10902 [Abstract] [Full Text]  
• Nuesch, J. P. F., Corbau, R., Tattersall, P., Rommelaere, J. (1998). Biochemical Activities of Minute Virus of Mice Nonstructural Protein NS1 Are Modulated In Vitro by the Phosphorylation State of the Polypeptide. J. Virol. 72: 8002-8012 [Abstract] [Full Text]  
• Naeger, L K, Schoborg, R V, Zhao, Q, Tullis, G E, Pintel, D J (1992). Nonsense mutations inhibit splicing of MVM RNA in cis when they interrupt the reading frame of either exon of the final spliced product.. Genes Dev. 6: 1107-1119 [Abstract]  
• Cotmore, S., Tattersall, P (1984). Characterization and molecular cloning of a human parvovirus genome. Science 226: 1161-1165 [Abstract]