This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Burg, J L Articles by Daniell, E Search for Related Content PubMed PubMed Citation Articles by Burg, J L Articles by Daniell, E

 Previous Article  |  Next Article 

J Virol. 1983 June; 46(3): 749-755

Introduction of superhelical turns into DNA by adenoviral core proteins and chromatin assembly factors.

ABSTRACT

The interaction in vitro between adenoviral histone-like proteins and DNA in the presence of chromatin assembly factors was investigated. Viral core protein VII or its precursor pVII was incubated with DNA in the presence of an extract of HeLa cell chromatin, which mediates nucleosome assembly from histones and DNA. We have demonstrated that either protein can introduce superhelical turns into relaxed closed-circular DNA and that the presence of chromatin extract is necessary for the supertwisting effect. A greater density of superhelical turns was produced by pVII than by VII, but neither protein-DNA interaction resulted in the "physiological" amount of supertwisting produced by histones. The inhibition of histone-induced supercoiling by both proteins and the protection of turns in supertwisted starting material are also described. The nucleosome assembly factor, nucleoplasmin, fails to mediate the introduction of superhelical turns by VII or pVII.

This article has been cited by other articles:

• Xue, Y., Johnson, J. S., Ornelles, D. A., Lieberman, J., Engel, D. A. (2005). Adenovirus Protein VII Functions throughout Early Phase and Interacts with Cellular Proteins SET and pp32. J. Virol. 79: 2474-2483 [Abstract] [Full Text]