This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by van Ooyen, A J Articles by Nusse, R Search for Related Content PubMed PubMed Citation Articles by van Ooyen, A J Articles by Nusse, R

 Previous Article  |  Next Article 

J Virol. 1983 May; 46(2): 362-370

Structural analysis of a 1.7-kilobase mouse mammary tumor virus-specific RNA.

ABSTRACT

We have detected a mouse mammary tumor virus (MMTV)-specific 1.7-kilobase (kb) polyadenylated RNA in mammary glands of several mouse strains. In BALB/c mice, it is the only MMTV-specific RNA species present. C3H and GR mammary glands and tumors contain, in addition, 3.8- and 7.8-kb MMTV RNAs. Nuclease S1 analysis was performed to map 1.7-kb polyadenylated RNA. It contains predominantly long terminal repeat (LTR) sequences. The 5' end maps approximately 134 nucleotides upstream from the 3' end of the LTR. Colinearity with complete proviral DNA continues to a site about 153 nucleotides downstream from the left (5') LTR. No sequences from the middle part of proviral DNA were found. Colinearity with proviral DNA is resumed 72 nucleotides upstream from the right (3') LTR. The nucleotide sequence in this area is TTCCAGT, which is a splice acceptor consensus sequence. The anatomy of 1.7-kb RNA indicates that it may serve as a messenger for the 36,700-dalton protein encoded by the LTRs of MMTV.

This article has been cited by other articles:

• Sen, N., Simmons, W. J., Thomas, R. M., Erianne, G., Zhang, D.-J., Jaeggli, N. S., Huang, C., Xiong, X., Tsiagbe, V. K., Ponzio, N. M., Thorbecke, G. J. (2001). META-Controlled env-Initiated Transcripts Encoding Superantigens of Murine Mtv29 and Mtv7 and Their Possible Role in B Cell Lymphomagenesis. J. Immunol. 166: 5422-5429 [Abstract] [Full Text]  
• Mustafa, F., Lozano, M., Dudley, J. P. (2000). C3H Mouse Mammary Tumor Virus Superantigen Function Requires a Splice Donor Site in the Envelope Gene. J. Virol. 74: 9431-9440 [Abstract] [Full Text]  
• Barnett, A., Mustafa, F., Wrona, T. J., Lozano, M., Dudley, J. P. (1999). Expression of Mouse Mammary Tumor Virus Superantigen mRNA in the Thymus Correlates with Kinetics of Self-Reactive T-Cell Loss. J. Virol. 73: 6634-6645 [Abstract] [Full Text]  
• Reuss, F. U., Coffin, J. M. (1998). Mouse Mammary Tumor Virus Superantigen Expression in B Cells Is Regulated by a Central Enhancer within the pol Gene. J. Virol. 72: 6073-6082 [Abstract] [Full Text]