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J Virol. 1983 March; 45(3): 1140-1142
Copyright © 1983, American Society for Microbiology. All Rights Reserved.

Reexamination of the Morphogenetic Block of a Putative Late-Stage, Temperature-Sensitive Mutant of Moloney Murine Leukemia Virus

Anthony Demsey^1, and Robert M. Friedman²

¹ Laboratory of Experimental Pathology, National Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases, Bethesda, Maryland 20205
² Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20014

ABSTRACT

The ts3 temperature-sensitive mutant of Moloney murine leukemia virus has been reported to have a morphogenetic block in a late stage of the budding process. As evidence, previously published electron micrographs of cells maintained at the nonpermissive temperature (39°C) revealed numerous budding virions on the cell surface. However, it appears now that these micrographs reflected budding that occurred not at 39°C, but after cells were removed from the incubator before fixation. The morphogenesis of ts3 is actually blocked at an earlier stage of development.

Present address: National Institute of General Medical Sciences, Bethesda, MD 20205.

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