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J Virol. 1982 December; 44(3): 767-771
Copyright © 1982, American Society for Microbiology. All Rights Reserved.

Two Unique RNA Species of the Nodavirus Black Beetle Virus

Jonathan P. Clewley^1,, W. Andrew L. Crump², Roger J. Avery¹ and Norman F. Moore²

¹ Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, England
² Natural Environment Research Council Institute of Virology, Oxford OX1 3SR, England

ABSTRACT

Two-dimensional fingerprinting of RNase T₁-derived oligonucleotides of the two individual RNA segments of the Nodavirus black beetle virus indicates that each RNA species possesses a distinct nucleotide sequence. Species 1 RNA has a genome complexity of approximately 3,000 nucleotides, and species 2 RNA is composed of approximately 1,500 nucleotides. Submolar amounts of oligonucleotides apparently derived from a third virus-specific RNA were also detected in black beetle virus RNA preparations.

Present address: Central Public Health Laboratory, Virus Reference Laboratory, London NW9 5HT, England.