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J Virol. 1982 December; 44(3): 1084-1087

DNA-binding properties of a herpes simplex virus immediate early protein.

ABSTRACT

The herpes simplex virus alpha, or immediate early, protein ICP4 has been shown to be central to the control of the early stages of virus replication. The detailed mechanism of this control is unknown. In this communication we show that purified ICP4 was unable to bind to DNA even though the protein was capable of such activity in a crude extract. Addition of either infected- or uninfected-cell extracts to the purified protein restored its DNA-binding activity. These results suggest that ICP4 binds to DNA only via a component of uninfected cells.

This article has been cited by other articles:

• Livingston, C. M., DeLuca, N. A., Wilkinson, D. E., Weller, S. K. (2008). Oligomerization of ICP4 and Rearrangement of Heat Shock Proteins May Be Important for Herpes Simplex Virus Type 1 Prereplicative Site Formation. J. Virol. 82: 6324-6336 [Abstract] [Full Text]  
• Kuddus, R. H., DeLuca, N. A. (2007). DNA-Dependent Oligomerization of Herpes Simplex Virus Type 1 Regulatory Protein ICP4. J. Virol. 81: 9230-9237 [Abstract] [Full Text]  
• Michael, N, Spector, D, Mavromara-Nazos, P, Kristie, T., Roizman, B (1988). The DNA-binding properties of the major regulatory protein alpha 4 of herpes simplex viruses. Science 239: 1531-1534 [Abstract]