This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Habara, A Articles by Aaronson, S A Search for Related Content PubMed PubMed Citation Articles by Habara, A Articles by Aaronson, S A

 Previous Article  |  Next Article 

J Virol. 1982 November; 44(2): 731-735

Rauscher murine leukemia virus: molecular cloning of infectious integrated proviral DNA.

ABSTRACT

The integrated proviral genome of Rauscher murine leukemia virus was molecularly cloned in a bacteriophage Charon 4A vector after the proviral sequences were enriched by sequential RPC-5 column chromatography and sucrose gradient centrifugation. A recombinant DNA clone, lambda-RV-1, possessing a 12-kilobase-pair EcoRI insert, was shown to contain the entire 8.8-kilobase-pair leukemia virus genome flanked by rat cellular sequences at the 5' and 3' ends. This DNA fragment was biologically active, inducing the release of virion-associated reverse transcriptase activity with as little as 10 ng of DNA insert. The virus induced XC plaque formation at high titers on NIH/3T3 and BALB/3T3 cells and demonstrated identity with the parental virus in radioimmunoassays for the highly type-specific gag gene-coded p12 protein. The molecularly cloned Rauscher murine leukemia virus should be useful in studying the molecular mechanisms involved in the transformation of specific lymphoid target cells by chronic mouse leukemia viruses.

This article has been cited by other articles:

• Chiu, I., Callahan, R, Tronick, SR, Schlom, J, Aaronson, S. (1984). Major pol gene progenitors in the evolution of oncoviruses. Science 223: 364-370 [Abstract]