This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Carroll, R B Articles by Gurney, E G Search for Related Content PubMed PubMed Citation Articles by Carroll, R B Articles by Gurney, E G

 Previous Article  |  Next Article 

J Virol. 1982 November; 44(2): 565-573

Time-dependent maturation of the simian virus 40 large T antigen-p53 complex studied by using monoclonal antibodies.

ABSTRACT

Newly synthesized simian virus 40 large tumor antigen (T Ag) slowly forms a stable complex with the host tumor antigen, "p53." By the use of immunological and temporal separations and inhibition of aggregation and processing by A locus mutation, we have distinguished specific steps in the reaction sequence leading to formation of the rapidly sedimenting oligomeric complex. The monoclonal antibody PAb101 bound only a fraction of the total soluble pulse-labeled T Ag bound by antitumor serum. After a chase, all T Ag had matured to the form recognized by PAb101. All p53 in the mouse line SVA31E7 was precipitated by the T Ag-specific monoclonal antibody PAb101, even after a short pulse, and is therefore entirely bound to mature T Ag. The p53-specific monoclonal antibody PAb122 precipitates nearly all of the mature T Ag recognized by PAb101, except A locus mutant T Ag, synthesized at the nonpermissive temperature. A locus mutation inhibited entry of newly synthesized T Ag into the oligomeric greater than 28S complex of T Ag and p53.

This article has been cited by other articles:

• Tyagarajan, S. K., Frisque, R. J. (2006). Stability and Function of JC Virus Large T Antigen and T' Proteins Are Altered by Mutation of Their Phosphorylated Threonine 125 Residues. J. Virol. 80: 2083-2091 [Abstract] [Full Text]  
• Manfredi, J. J., Dong, J., Liu, W.-j., Resnick-Silverman, L., Qiao, R., Chahinian, P., Saric, M., Gibbs, A. R., Phillips, J. I., Murray, J., Axten, C. W., Nolan, R. P., Aaronson, S. A. (2005). Evidence against a Role for SV40 in Human Mesothelioma. Cancer Res. 65: 2602-2609 [Abstract] [Full Text]  
• Cole, S. L., Tevethia, M. J. (2002). Simian Virus 40 Large T Antigen and Two Independent T-Antigen Segments Sensitize Cells to Apoptosis following Genotoxic Damage. J. Virol. 76: 8420-8432 [Abstract] [Full Text]  
• Liu, J.-L., Ye, Y., Qian, Z., Qian, Y., Templeton, D. J., Lee, L. F., Kung, H.-J. (1999). Functional Interactions between Herpesvirus Oncoprotein MEQ and Cell Cycle Regulator CDK2. J. Virol. 73: 4208-4219 [Abstract] [Full Text]