This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Iverson, L E Articles by Rose, J K Search for Related Content PubMed PubMed Citation Articles by Iverson, L E Articles by Rose, J K

Sequential synthesis of 5'-proximal vesicular stomatitis virus mRNA sequences.

ABSTRACT

We examined the kinetics of synthesis in vitro of the 5' ends of the vesicular stomatitis virus mRNAs by analysis of specific RNase T1 oligonucleotides located near the 5' ends of the mRNAs. Our results indicate that, like synthesis of full-length mRNAs, the 5' ends of the mRNAs are synthesized sequentially, following the gene order N, NS, and M. Additional experiments with UV-irradiated virus demonstrated that synthesis of the mRNA regions containing these oligonucleotides is dependent on synthesis of the mRNA from the preceding gene. These results are inconsistent with a model of vesicular stomatitis virus transcription involving simultaneous initiation and presynthesis of leader RNAs 30 to 70 nucleotides long for each mRNA. We also characterized two small RNA species whose synthesis is highly resistant to UV irradiation. Partial sequence analysis indicates that these RNAs are a 5'-capped fragment of the N mRNA and a 5' fragment of the leader RNA.

This article has been cited by other articles:

• Sun, M., Fuentes, S. M., Timani, K., Sun, D., Murphy, C., Lin, Y., August, A., Teng, M. N., He, B. (2008). Akt Plays a Critical Role in Replication of Nonsegmented Negative-Stranded RNA Viruses. J. Virol. 82: 105-114 [Abstract] [Full Text]  
• Carlos, T. S., Fearns, R., Randall, R. E. (2005). Interferon-Induced Alterations in the Pattern of Parainfluenza Virus 5 Transcription and Protein Synthesis and the Induction of Virus Inclusion Bodies. J. Virol. 79: 14112-14121 [Abstract] [Full Text]  
• Zhao, H., Peeters, B. P. H. (2003). Recombinant Newcastle disease virus as a viral vector: effect of genomic location of foreign gene on gene expression and virus replication. J. Gen. Virol. 84: 781-788 [Abstract] [Full Text]  
• Rodriguez, L. L., Pauszek, S. J., Bunch, T. A., Schumann, K. R. (2002). Full-length genome analysis of natural isolates of vesicular stomatitis virus (Indiana 1 serotype) from North, Central and South America. J. Gen. Virol. 83: 2475-2483 [Abstract] [Full Text]  
• Weik, M., Modrof, J., Klenk, H.-D., Becker, S., Muhlberger, E. (2002). Ebola Virus VP30-Mediated Transcription Is Regulated by RNA Secondary Structure Formation. J. Virol. 76: 8532-8539 [Abstract] [Full Text]  
• Le Mercier, P., Garcin, D., Hausmann, S., Kolakofsky, D. (2002). Ambisense Sendai Viruses Are Inherently Unstable but Are Useful To Study Viral RNA Synthesis. J. Virol. 76: 5492-5502 [Abstract] [Full Text]  
• Stillman, E. A., Whitt, M. A. (1999). Transcript Initiation and 5'-End Modifications Are Separable Events during Vesicular Stomatitis Virus Transcription. J. Virol. 73: 7199-7209 [Abstract] [Full Text]  
• He, B., Lamb, R. A. (1999). Effect of Inserting Paramyxovirus Simian Virus 5 Gene Junctions at the HN/L Gene Junction: Analysis of Accumulation of mRNAs Transcribed from Rescued Viable Viruses. J. Virol. 73: 6228-6234 [Abstract] [Full Text]  
• Stillman, E. A., Whitt, M. A. (1998). The Length and Sequence Composition of Vesicular Stomatitis Virus Intergenic Regions Affect mRNA Levels and the Site of Transcript Initiation. J. Virol. 72: 5565-5572 [Abstract] [Full Text]