This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gaynor, R B Articles by Berk, A J Search for Related Content PubMed PubMed Citation Articles by Gaynor, R B Articles by Berk, A J

 Previous Article  |  Next Article 

J Virol. 1982 October; 44(1): 276-285

Enhanced expression of adenovirus transforming proteins.

ABSTRACT

Proteins encoded in regions EIA and EIB of human adenoviruses cause transformation of rodent cells. One protein from EIA also stimulates transcription of other early regions at early times in a productive infection. In the past, direct analysis of these proteins synthesized in vivo has been difficult because of the low levels produced in both transformed cells and productively infected cells. We present a simple method which leads to expression of EIA and EIB mRNAs and proteins at 30-fold greater levels than those observed during the early phase of a standard productive infection. Under these conditions, these proteins are among the most prominent translation products of infected cells. This allowed direct visualization of EIA and EIB proteins on two-dimensional gels of pulse-labeled total cell protein. Experiments with EIA and EIB mutants confirm that the identified proteins are indeed encoded in these regions. Two EIA proteins are observed, one translated from each of the major early EIA mRNAs. Both of these EIA proteins are phosphorylated.

This article has been cited by other articles:

• Chen, I., Cann, A., Shah, N., Gaynor, R. (1985). Functional relation between HTLV-II x and adenovirus E1A proteins in transcriptional activation. Science 230: 570-573 [Abstract]  
• Gaynor, R., Feldman, L., Berk, A. (1985). Transcription of class III genes activated by viral immediate early proteins. Science 230: 447-450 [Abstract]