This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sabara, M Articles by Misra, V Search for Related Content PubMed PubMed Citation Articles by Sabara, M Articles by Misra, V

Effect of tunicamycin on rotavirus assembly and infectivity.

ABSTRACT

Bovine rotavirus grown in the presence or absence of tunicamycin was analyzed with respect to yield of infectious virus, the ratio of complete to incomplete particles, and polypeptide composition. Tunicamycin at a concentration of 1 microgram/ml reduced virus yields by 4 logs and completely prevented the incorporation of [3H]uridine into complete rotavirus particles, as determined by cesium chloride gradient analysis. Concomitant with a reduction in complete particles, three rotavirus polypeptides shifted in their relative position on polyacrylamide gels from 41,900-molecular-weight position (41.9K), 29.3K, and 16.1K to migrate at 35.5K, 22.7K, and 15.5K, respectively. Limited proteolysis indicated that the lower-molecular-weight polypeptides possessed the same constituent peptides as the larger polypeptides, suggesting that they represented the unglycosylated equivalents. These results suggest that interference with glycosylation prevents proper assembly of the outer coat proteins in bovine rotavirus.

This article has been cited by other articles:

• Cuadras, M. A., Bordier, B. B., Zambrano, J. L., Ludert, J. E., Greenberg, H. B. (2006). Dissecting Rotavirus Particle-Raft Interaction with Small Interfering RNAs: Insights into Rotavirus Transit through the Secretory Pathway.. J. Virol. 80: 3935-3946 [Abstract] [Full Text]  
• Delmas, O., Durand-Schneider, A.-M., Cohen, J., Colard, O., Trugnan, G. (2004). Spike Protein VP4 Assembly with Maturing Rotavirus Requires a Postendoplasmic Reticulum Event in Polarized Caco-2 Cells. J. Virol. 78: 10987-10994 [Abstract] [Full Text]  
• Mirazimi, A., Svensson, L. (1998). Carbohydrates Facilitate Correct Disulfide Bond Formation and Folding of Rotavirus VP7. J. Virol. 72: 3887-3892 [Abstract] [Full Text]