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J Virol. 1982 July; 43(1): 365-367

In vitro packaging of bacteriophage T7 DNA requires ATP.

W E Masker

ABSTRACT

Removal of nucleoside triphosphates from extracts prepared from bacteriophage T7-infected Escherichia coli results in a stringent requirement for added ATP to form infective phage particles by in vitro packaging of bacteriophage T7 DNA. Optimal packaging efficiency was achieved at a concentration of about 1.25 mM. Other nucleoside triphosphates could be substituted for ATP, but none of the common nucleoside triphosphates was as effective as ATP in promoting in vitro encapsulation.

J Virol. 1982 July; 43(1): 365-367




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