This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Furth, M E
Right arrow Articles by Scolnick, E M
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Furth, M E
Right arrow Articles by Scolnick, E M

 Previous Article  |  Next Article 

J Virol. 1982 July; 43(1): 294-304

Monoclonal antibodies to the p21 products of the transforming gene of Harvey murine sarcoma virus and of the cellular ras gene family.

M E Furth, L J Davis, B Fleurdelys and E M Scolnick

ABSTRACT

We have isolated eight rat lymphocyte-myeloma hybrid cell lines producing monoclonal antibodies that react with the 21,000-dalton transforming protein (p21) encoded by the v-ras gene of Harvey murine sarcoma virus (Ha-MuSV). These antibodies specifically immunoprecipitate both phosphorylated and non-phosphorylated forms of p21 from lysates of cells transformed by Ha-MuSV. All eight react with the products of closely related ras genes expressed in cells transformed by two additional sarcoma viruses (rat sarcoma virus and BALB sarcoma virus) or by a cellular Harvey-ras gene placed under the control of a viral promoter. Three of the antibodies also react strongly with the p21 encoded by the v-ras gene of Kirsten MuSV. These same three antibodies immunoprecipitate the predominant p21 species synthesized normally in a variety of rodent cell lines, including the p21 produced at high levels in 416B murine hemopoietic cells. This suggests that an endogenous gene closely related to Kirsten-ras is expressed in these cells. The monoclonal antibodies have been used to confirm two properties associated with p21; localization at the inner surface of the membrane of Ha-MuSV-transformed cells, assayed by immunofluorescence microscopy, and binding of guanine nucleotides.


J Virol. 1982 July; 43(1): 294-304




This article has been cited by other articles:

  • Watts, G. M., Beurskens, F. J. M., Martin-Padura, I., Ballantyne, C. M., Klickstein, L. B., Brenner, M. B., Lee, D. M. (2005). Manifestations of Inflammatory Arthritis Are Critically Dependent on LFA-1. J. Immunol. 174: 3668-3675 [Abstract] [Full Text]  
  • Perez de Castro, I., Diaz, R., Malumbres, M., Hernandez, M.-I., Jagirdar, J., Jimenez, M., Ahn, D., Pellicer, A. (2003). Mice Deficient for N-ras: Impaired Antiviral Immune Response and T-Cell Function. Cancer Res. 63: 1615-1622 [Abstract] [Full Text]  
  • Silver, E. T., Lavender, K. J., Gong, D.-E., Hazes, B., Kane, K. P. (2002). Allelic Variation in the Ectodomain of the Inhibitory Ly-49G2 Receptor Alters Its Specificity for Allogeneic and Xenogeneic Ligands. J. Immunol. 169: 4752-4760 [Abstract] [Full Text]  
  • Dumaz, N., Light, Y., Marais, R. (2002). Cyclic AMP Blocks Cell Growth through Raf-1-Dependent and Raf-1-Independent Mechanisms. Mol. Cell. Biol. 22: 3717-3728 [Abstract] [Full Text]  
  • D'Abaco, G. M., Hooper, S., Paterson, H., Marshall, C. J. (2002). Loss of Rb overrides the requirement for ERK activity for cell proliferation. J. Cell Sci. 115: 4607-4616 [Abstract] [Full Text]  
  • Self, A., Caron, E, Paterson, H., Hall, A (2001). Analysis of R-Ras signalling pathways. J. Cell Sci. 114: 1357-1366 [Abstract]  
  • Ammit, A. J., Kane, S. A., Panettieri, R. A. Jr. (1999). Activation of K-p21ras and N-p21ras, but Not H-p21ras, Is Necessary for Mitogen-Induced Human Airway Smooth-Muscle Proliferation. Am. J. Respir. Cell Mol. Bio. 21: 719-727 [Abstract] [Full Text]  
  • Woo, P. L., Ching, D., Guan, Y., Firestone, G. L. (1999). Requirement for Ras and Phosphatidylinositol 3-Kinase Signaling Uncouples the Glucocorticoid-induced Junctional Organization and Transepithelial Electrical Resistance in Mammary Tumor Cells. J. Biol. Chem. 274: 32818-32828 [Abstract] [Full Text]  
  • Chiloeches, A., Paterson, H. F., Marais, R., Clerk, A., Marshall, C. J., Sugden, P. H. (1999). Regulation of Ras{middle dot}GTP Loading and Ras-Raf Association in Neonatal Rat Ventricular Myocytes by G Protein-coupled Receptor Agonists and Phorbol Ester. ACTIVATION OF THE EXTRACELLULAR SIGNAL-REGULATED KINASE CASCADE BY PHORBOL ESTER IS MEDIATED BY Ras. J. Biol. Chem. 274: 19762-19770 [Abstract] [Full Text]  
  • Anborgh, P. H., Qian, X., Papageorge, A. G., Vass, W. C., DeClue, J. E., Lowy, D. R. (1999). Ras-Specific Exchange Factor GRF: Oligomerization through Its Dbl Homology Domain and Calcium-Dependent Activation of Raf. Mol. Cell. Biol. 19: 4611-4622 [Abstract] [Full Text]  
  • Nobes, C. D., Hall, A. (1999). Rho GTPases Control Polarity, Protrusion, and Adhesion during Cell Movement. JCB 144: 1235-1244 [Abstract] [Full Text]  
  • Walker, F., Kato, A., Gonez, L. J., Hibbs, M. L., Pouliot, N., Levitzki, A., Burgess, A. W. (1998). Activation of the Ras/Mitogen-Activated Protein Kinase Pathway by Kinase-Defective Epidermal Growth Factor Receptors Results in Cell Survival but Not Proliferation. Mol. Cell. Biol. 18: 7192-7204 [Abstract] [Full Text]  
  • Leblanc, V., Tocque, B., Delumeau, I. (1998). Ras-GAP Controls Rho-Mediated Cytoskeletal Reorganization through Its SH3 Domain. Mol. Cell. Biol. 18: 5567-5578 [Abstract] [Full Text]  
  • Punch, J. D., Tono, T., Qin, L., Bishop, D. K., Bromberg, J. S. (1998). Tolerance Induction by Anti-CD2 Plus Anti-CD3 Monoclonal Antibodies: Evidence for an IL-4 Requirement. J. Immunol. 161: 1156-1162 [Abstract] [Full Text]  
  • Corbley, M. J. (1997). Transformation by Ras Suppresses Expression of the Neurotrophic Growth Factor Pleiotrophin. J. Biol. Chem. 272: 24696-24702 [Abstract] [Full Text]  
  • Tuxworth, R. I., Cheetham, J. L., Machesky, L. M., Spiegelmann, G. B., Weeks, G., Insall, R. H. (1997). Dictyostelium RasG Is Required for Normal Motility and Cytokinesis, But Not Growth. JCB 138: 605-614 [Abstract] [Full Text]  
  • Tinhofer, I., Maly, K., Dietl, P., Hochholdinger, F., Mayr, S., Obermeier, A., Grunicke, H. H. (1996). Differential Ca2+ Signaling Induced by Activation of the Epidermal Growth Factor and Nerve Growth Factor Receptors. J. Biol. Chem. 271: 30505-30509 [Abstract] [Full Text]  
  • Ernst, M., Oates, A., Dunn, A. R. (1996). gp130-mediated Signal Transduction in Embryonic Stem Cells Involves Activation of Jak and Ras/Mitogen-activated Protein Kinase Pathways. J. Biol. Chem. 271: 30136-30143 [Abstract] [Full Text]  
  • Chen, G., Oh, S., Monia, B. P., Stacey, D. W. (1996). Antisense Oligonucleotides Demonstrate a Dominant Role of c-Ki-RAS Proteins in Regulating the Proliferation of Diploid Human Fibroblasts. J. Biol. Chem. 271: 28259-28265 [Abstract] [Full Text]  
  • Gardner, A. M., Johnson, G. L. (1996). Fibroblast Growth Factor-2 Suppression of Tumor Necrosis Factor alpha -Mediated Apoptosis Requires Ras and the Activation of Mitogen-activated Protein Kinase. J. Biol. Chem. 271: 14560-14566 [Abstract] [Full Text]  
  • Knall, C., Young, S., Nick, J. A., Buhl, A. M., Worthen, G. S., Johnson, G. L. (1996). Interleukin-8 Regulation of the Ras/Raf/Mitogen-activated Protein Kinase Pathway in Human Neutrophils. J. Biol. Chem. 271: 2832-2838 [Abstract] [Full Text]  
  • Lander, H. M., Ogiste, J. S., Levi, R., Novogrodsky, A. (1995). Nitric Oxide-stimulated Guanine Nucleotide Exchange on p21[IMAGE]. J. Biol. Chem. 270: 7017-7020 [Abstract] [Full Text]  
  • Jiang, H., Lu, Z., Luo, J.-Q., Wolfman, A., Foster, D. A. (1995). Ras Mediates the Activation of Phospholipase D by v-Src. J. Biol. Chem. 270: 6006-6009 [Abstract] [Full Text]  
  • Serrano, M, Gomez-Lahoz, E, DePinho, R., Beach, D, Bar-Sagi, D (1995). Inhibition of ras-induced proliferation and cellular transformation by p16INK4. Science 267: 249-252 [Abstract]  
  • Sun, H, Tonks, N., Bar-Sagi, D (1994). Inhibition of Ras-induced DNA synthesis by expression of the phosphatase MKP-1. Science 266: 285-288 [Abstract]  
  • Kohl, N., Mosser, S., deSolms, S., Giuliani, E., Pompliano, D., Graham, S., Smith, R., Scolnick, E., Oliff, A, Gibbs, J. (1993). Selective inhibition of ras-dependent transformation by a farnesyltransferase inhibitor. Science 260: 1934-1937 [Abstract]  
  • Lazaris-Karatzas, A, Smith, M R, Frederickson, R M, Jaramillo, M L, Liu, Y L, Kung, H F, Sonenberg, N (1992). Ras mediates translation initiation factor 4E-induced malignant transformation.. Genes Dev. 6: 1631-1642 [Abstract]  
  • Zhang, K, Papageorge, A., Lowy, D. (1992). Mechanistic aspects of signaling through Ras in NIH 3T3 cells. Science 257: 671-674 [Abstract]  
  • Zhang, K, Papageorge, A., Martin, P, Vass, W., Olah, Z, Polakis, P., McCormick, F, Lowy, D. (1991). Heterogeneous amino acids in Ras and Rap1A specifying sensitivity to GAP proteins. Science 254: 1630-1634 [Abstract]  
  • Schafer, W., Trueblood, C., Yang, C., Mayer, M., Rosenberg, S, Poulter, C., Kim, S., Rine, J (1990). Enzymatic coupling of cholesterol intermediates to a mating pheromone precursor and to the ras protein. Science 249: 1133-1139 [Abstract]  
  • Schafer, W., Kim, R, Sterne, R, Thorner, J, Kim, S., Rine, J (1989). Genetic and pharmacological suppression of oncogenic mutations in ras genes of yeast and humans. Science 245: 379-385 [Abstract]  
  • Adari, H, Lowy, D., Willumsen, B., Der, C., McCormick, F (1988). Guanosine triphosphatase activating protein (GAP) interacts with the p21 ras effector binding domain. Science 240: 518-521 [Abstract]  
  • Sukumar, S, Carney, W., Barbacid, M (1988). Independent molecular pathways in initiation and loss of hormone responsiveness of breast carcinomas. Science 240: 524-526 [Abstract]  
  • de Vos, A., Tong, L, Milburn, M., Matias, P., Jancarik, J, Noguchi, S, Nishimura, S, Miura, K, Ohtsuka, E, Kim, S. (1988). Three-dimensional structure of an oncogene protein: catalytic domain of human c-H-ras p21. Science 239: 888-893 [Abstract]  
  • Kim, S.-H., de Vos, A.M., Tong, L., Milburn, M.V., Matias, P.M., Jancarik, J., Ohtsuka, E., Nishimura, S. (1988). ras Oncogene Proteins: Three-dimensional Structures, Functional Implications, and a Model for Signal Transducer. Cold Spring Harb Symp Quant Biol 53: 273-281 [Abstract]  
  • Hall, A., Cales, C., Hancock, J.F., Lloyd, A., Self, A., Gardener, S., Houslay, M.D., Wakelam, M.J.O., Marshall, C.J. (1988). Analysis of Mammalian ras Effector Function. Cold Spring Harb Symp Quant Biol 53: 855-862 [Abstract]  
  • Stacey, D.W., Tsai, M.-H., Yu, C.-L., Smith, J.K. (1988). Critical Role of Cellular ras Proteins in Proliferative Signal Transduction. Cold Spring Harb Symp Quant Biol 53: 871-881 [Abstract]  
  • Downward, J., Weinberg, R.A. (1988). Influence of p21ras on Phosphatidylinositol Turnover. Cold Spring Harb Symp Quant Biol 53: 883-892 [Abstract]  
  • KOEFFLER, H. P. (1987). Syndromes of Acute Nonlymphocytic Leukemia. ANN INTERN MED 107: 748-758 [Abstract]  
  • Lacal, J., de la Pena, P, Moscat, J, Garcia-Barreno, P, Anderson, P., Aaronson, S. (1987). Rapid stimulation of diacylglycerol production in Xenopus oocytes by microinjection of H-ras p21. Science 238: 533-536 [Abstract]  
  • Trahey, M, McCormick, F (1987). A cytoplasmic protein stimulates normal N-ras p21 GTPase, but does not affect oncogenic mutants. Science 238: 542-545 [Abstract]  
  • Reynolds, S., Stowers, S., Patterson, R., Maronpot, R., Aaronson, S., Anderson, M. (1987). Activated oncogenes in B6C3F1 mouse liver tumors: implications for risk assessment. Science 237: 1309-1316 [Abstract]  
  • Rhim, J., Fujita, J, Arnstein, P, Aaronson, S. (1986). Neoplastic conversion of human keratinocytes by adenovirus 12-SV40 virus and chemical carcinogens. Science 232: 385-388 [Abstract]  
  • Fleischman, L., Chahwala, S., Cantley, L (1986). ras-transformed cells: altered levels of phosphatidylinositol-4,5-bisphosphate and catabolites. Science 231: 407-410 [Abstract]  
  • Androphy, E., Schiller, J., Lowy, D. (1985). Identification of the protein encoded by the E6 transforming gene of bovine papillomavirus. Science 230: 442-445 [Abstract]  
  • Kasid, A, Lippman, M., Papageorge, A., Lowy, D., Gelmann, E. (1985). Transfection of v-rasH DNA into MCF-7 human breast cancer cells bypasses dependence on estrogen for tumorigenicity. Science 228: 725-728 [Abstract]  
  • DeFeo-Jones, D, Tatchell, K, Robinson, L., Sigal, I., Vass, W., Lowy, D., Scolnick, E. (1985). Mammalian and yeast ras gene products: biological function in their heterologous systems. Science 228: 179-184 [Abstract]  
  • Yoakum, G., Lechner, J., Gabrielson, E., Korba, B., Malan-Shibley, L, Willey, J., Valerio, M., Shamsuddin, A., Trump, B., Harris, C. (1985). Transformation of human bronchial epithelial cells transfected by Harvey ras oncogene. Science 227: 1174-1179 [Abstract]  
  • Feig, L., Bast, R. Jr, Knapp, R., Cooper, G. (1984). Somatic activation of rasK gene in a human ovarian carcinoma. Science 223: 698-701 [Abstract]  
  • Needleman, S., Yuasa, Y, Srivastava, S, Aaronson, S. (1983). Normal cells of patients with high cancer risk syndromes lack transforming activity in the NIH/3T3 transfection assay. Science 222: 173-175 [Abstract]  
  • Lautenberger, J., Ulsh, L, Shih, T., Papas, T. (1983). High-level expression in Escherichia coli of enzymatically active Harvey murine sarcoma virus p21ras protein. Science 221: 858-860 [Abstract]