This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Jenkins, N A Articles by Lee, B K Search for Related Content PubMed PubMed Citation Articles by Jenkins, N A Articles by Lee, B K

Ecotropic murine leukemia virus DNA content of normal and lymphomatous tissues of BXH-2 recombinant inbred mice.

ABSTRACT

BXH-2 recombinant inbred mice spontaneously produce a B-tropic murine leukemia virus (MuLV) beginning early in life and have a high incidence of non-T-cell lymphomas. These traits are not characteristic of the progenitor strains (C57BL/6J and C3H/HeJ) or of 11 other BXH recombinant inbred strains. Since B-tropic virus expression may be causally related to the high incidence of lymphoma in this strain, we have analyzed the ecotropic MuLV DNA content of both normal and lymphomatous tissues of BXH-2 mice. Southern analysis and hybridization with an ecotropic MuLV DNA-specific probe showed that DNA of normal BXH-2 tissues contained both parental N-tropic MuLV proviruses but lacked endogenous B-tropic MuLV DNA sequences. In addition, none of 116 F1 hybrid mice derived from male BXH-2 mice spontaneously produced ecotropic MuLV early in life. These results suggest that the B-tropic virus is horizontally transmitted in BXH-2 mice. Southern analysis of DNA from tumor tissues of 12 BXH-2 mice showed that amplification of ecotropic-specific DNA sequences had occurred in lymphomatous tissues of 3 mice and suggested that these tumors were monoclonal. The number of newly acquired proviruses, which appeared to be structurally nondefective and integrated at different sites, varied from one to three copies. Since lymphomatous tissues from only 3 of 12 mice examined carried additional detectable ecotropic proviruses, these results suggest that amplification of ecotropic MuLV DNA sequences is not required for maintenance of transformation in BXH-2 lymphomas.

This article has been cited by other articles:

• Kawamura-Saito, M., Yamazaki, Y., Kaneko, K., Kawaguchi, N., Kanda, H., Mukai, H., Gotoh, T., Motoi, T., Fukayama, M., Aburatani, H., Takizawa, T., Nakamura, T. (2006). Fusion between CIC and DUX4 up-regulates PEA3 family genes in Ewing-like sarcomas with t(4;19)(q35;q13) translocation. Hum Mol Genet 15: 2125-2137 [Abstract] [Full Text]  
• Carlson, C. M., Frandsen, J. L., Kirchhof, N., McIvor, R. S., Largaespada, D. A. (2005). Somatic integration of an oncogene-harboring Sleeping Beauty transposon models liver tumor development in the mouse. Proc. Natl. Acad. Sci. USA 102: 17059-17064 [Abstract] [Full Text]  
• Turcotte, K., Gauthier, S., Tuite, A., Mullick, A., Malo, D., Gros, P. (2005). A mutation in the Icsbp1 gene causes susceptibility to infection and a chronic myeloid leukemia-like syndrome in BXH-2 mice. J. Exp. Med. 201: 881-890 [Abstract] [Full Text]  
• Iwasaki, M., Kuwata, T., Yamazaki, Y., Jenkins, N. A., Copeland, N. G., Osato, M., Ito, Y., Kroon, E., Sauvageau, G., Nakamura, T. (2005). Identification of cooperative genes for NUP98-HOXA9 in myeloid leukemogenesis using a mouse model. Blood 105: 784-793 [Abstract] [Full Text]  
• O'Sullivan, T. N., Wu, X. S., Rachel, R. A., Huang, J.-D., Swing, D. A., Matesic, L. E., Hammer, J. A. III, Copeland, N. G., Jenkins, N. A. (2004). dsu functions in a MYO5A-independent pathway to suppress the coat color of dilute mice. Proc. Natl. Acad. Sci. USA 101: 16831-16836 [Abstract] [Full Text]  
• Turcotte, K., Gauthier, S., Mitsos, L.-M., Shustik, C., Copeland, N. G., Jenkins, N. A., Fournet, J.-C., Jolicoeur, P., Gros, P. (2004). Genetic control of myeloproliferation in BXH-2 mice. Blood 103: 2343-2350 [Abstract] [Full Text]  
• Carlson, C. M., Dupuy, A. J., Fritz, S., Roberg-Perez, K. J., Fletcher, C. F., Largaespada, D. A. (2003). Transposon Mutagenesis of the Mouse Germline. Genetics 165: 243-256 [Abstract] [Full Text]  
• Himmel, K. L., Bi, F., Shen, H., Jenkins, N. A., Copeland, N. G., Zheng, Y., Largaespada, D. A. (2002). Activation of Clg, a Novel Dbl Family Guanine Nucleotide Exchange Factor Gene, by Proviral Insertion at Evi24, a Common Integration Site in B Cell and Myeloid Leukemias. J. Biol. Chem. 277: 13463-13472 [Abstract] [Full Text]  
• Dupuy, A. J., Clark, K., Carlson, C. M., Fritz, S., Davidson, A. E., Markley, K. M., Finley, K., Fletcher, C. F., Ekker, S. C., Hackett, P. B., Horn, S., Largaespada, D. A. (2002). Mammalian germ-line transgenesis by transposition. Proc. Natl. Acad. Sci. USA 99: 4495-4499 [Abstract] [Full Text]  
• Fujino, T., Suzuki, A., Ito, Y., Ohyashiki, K., Hatano, Y., Miura, I., Nakamura, T. (2002). Single-translocation and double-chimeric transcripts: detection of NUP98-HOXA9 in myeloid leukemias with HOXA11 or HOXA13 breaks of the chromosomal translocation t(7;11)(p15;p15). Blood 99: 1428-1433 [Abstract] [Full Text]  
• Nakamura, T., Yamazaki, Y., Hatano, Y., Miura, I. (1999). NUP98 Is Fused to PMX1 Homeobox Gene in Human Acute Myelogenous Leukemia With Chromosome Translocation t(1;11)(q23;p15). Blood 94: 741-747 [Abstract] [Full Text]  
• Joyner, A L, Martin, G R (1987). En-1 and En-2, two mouse genes with sequence homology to the Drosophila engrailed gene: expression during embryogenesis.. Genes Dev. 1: 29-38 [Abstract]  
• Dupuy, A. J., Morgan, K., von Lintig, F. C., Shen, H., Acar, H., Hasz, D. E., Jenkins, N. A., Copeland, N. G., Boss, G. R., Largaespada, D. A. (2001). Activation of the Rap1 Guanine Nucleotide Exchange Gene, CalDAG-GEF I, in BXH-2 Murine Myeloid Leukemia. J. Biol. Chem. 276: 11804-11811 [Abstract] [Full Text]  
• Dupuy, A. J., Clark, K., Carlson, C. M., Fritz, S., Davidson, A. E., Markley, K. M., Finley, K., Fletcher, C. F., Ekker, S. C., Hackett, P. B., Horn, S., Largaespada, D. A. (2002). Mammalian germ-line transgenesis by transposition. Proc. Natl. Acad. Sci. USA 99: 4495-4499 [Abstract] [Full Text]