This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Warocquier, R. Articles by Green, M. Search for Related Content PubMed PubMed Citation Articles by Warocquier, R. Articles by Green, M.

 Previous Article  |  Next Article 

J Virol. 1969 October; 4(4): 423-428
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Biochemical Studies on Adenovirus Multiplication

XVI. Transcription of the Adenovirus Genome During Abortive Infection at Elevated Temperatures

Institute for Molecular Virology, Saint Louis University School of Medicine, Saint Louis, Missouri 63104
Institut Pasteur, Lille 59, France

ABSTRACT

The synthesis of infectious virus is reduced by 99% in adenovirus type 2-infected KB cells maintained at 42 C in suspension. Studies to delineate the steps in virus biosynthesis blocked at 42 C revealed the following. (i) The inhibition of ribosomal ribonucleic acid (RNA) synthesis and the block in the conversion of ribosomal RNA precursors to ribosomal RNA which normally occur in infected cells at 37 C was accentuated at 42 C. (ii) The same species of early and late viral messenger RNA were synthesized at 37 and 42 C. (iii) The shift from host cell deoxyribonucleic acid (DNA) synthesis to viral DNA replication occurred earlier at 42 C than at 37 C. These findings indicate that the thermosensitive block(s) in virion formation does not occur at the level of viral DNA replication or transcription of viral mRNA but probably involves the synthesis of late viral proteins or the maturation of the virion.

¹ Permanent address: Institut Pasteur, Lille 59, France.

² Research Career Awardee of the National Institutes of Health (5-K6-AI-4739).