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J Virol. 1969 October; 4(4): 372-379
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Reaction of Chick Embryo Cells Infected with Leukosis Strain MC29 Virus with Fluorescein-Labeled Antibody

Robert B. Fritz, A. J. Langlois and J. W. Beard

Department of Surgery, Duke University Medical Center, Durham, North Carolina 27706

ABSTRACT

Immune serum was prepared in the rabbit with BAI strain A leukosis virus isolated by centrifugal fractionation from the plasma of chickens with myeloblastic leukemia and further purified on a potassium tartrate gradient. Antibody to group-specific antigen was demonstrated in the serum by immunoelectrophoresis and immunodiffusion. Fluorescein-conjugated serum was used unabsorbed and absorbed with chick cells for study of acetone-fixed chick embryo cells uninfected or infected with strain MC29 avian leukosis virus. With unabsorbed serum, large numbers of cytoplasmic particles stained in a few cells within 2 hr after exposure to virus, and the cell number increased greatly in 24 hr. Absorption of the serum abolished the early reaction. Staining with absorbed serum was delayed until about 14 hr after culture exposure to virus, but essentially all cells were stained within 72 hr at the time when all cells were morphologically altered. Differences between the responses to unabsorbed and absorbed serum suggested cytoplasmic formation or concentration of chick tissue antigen similar to that incorporated in leukosis virus particles. The characteristics of staining with absorbed serum were similar to those observed by others in analogous studies with avian tumor viruses.

J Virol. 1969 October; 4(4): 372-379
Copyright © 1969 American Society for Microbiology. All Rights Reserved.





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Copyright © 1969 by the American Society for Microbiology. All rights reserved.