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J Virol. 1969 October; 4(4): 339-347
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Biophysical Properties of Frog Virus and Its Deoxyribonucleic Acid: Fate of Radioactive Virus in the Early Stage of Infection ¹

Department of Microbiology, University of California Medical Center, San Francisco, California 94122

ABSTRACT

Frog virus (FV-3) was banded by isopycnic centrifugation in cesium chloride, sucrose, or potassium tartrate. Two bands of infectivity were regularly found at positions in cesium chloride corresponding to densities of 1.26 and 1.30 g/cm³, respectively. Deoxyribonucleic acid from either band had the following characteristics: double-stranded; a T_m of 76.3 C in 0.1 SSC (0.015 M NaCl plus 0.015 M sodium citrate) and a buoyant density of 1.720 g/cm³ in cesium chloride, corresponding to a guanine plus cytosine content of 56 to 58% and a molecular weight of 130 x 10⁶ daltons, determined by velocity sedimentation. These data, together with electron micrographs of sections of cells infected with material from either band suggest that two types of infectious frog virus particles exists, rather than a second virus in the frog virus stocks. The composition of frog virus was determined. It was found that highly purified preparations of frog virus were composed of 55.8% protein, 30.1% deoxyribonucleic acid, and 14.2% lipid. The kinetics of adsorption and uncoating of FV-3 was studied with radioactive virus. Uncoating is comparatively rapid and in contrast to poxvirus is unaffected by inhibitors of protein synthesis.

² Present address: Roche Institute for Molecular Biology, Nutley, N.J. 07110.

¹ Contains material presented in partial fulfillment of the requirements for the Ph.D. degree from the Department of Microbiology of the University of Tennessee Medical Units, Memphis, Tenn.