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J Virol. 1969 October; 4(4): 317-322
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Inactivation of Bacteriophage Lambda Containing Semiconserved Alkylated Deoxyribonucleic Acid

Laboratory of Genetics, Hebrew University of Jerusalem, Israel

ABSTRACT

Immediate and delayed inactivation of ethylmethane sulfonate (EMS)-treated phage were studied. Phage particles with one alkylated and one intact deoxyribonucleic acid (DNA) strand were obtained by allowing host-modified, EMS-treated phage to undergo one growth cycle in a nonmodifying host and selecting the progeny with semiconserved parental DNA on a restricting host. The results indicate that particles with one alkylated DNA strand are more sensitive to a second treatment with the alkylating agent. When incubated at 37 C, they are subject to inactivation at a rate which is smaller than that of phages containing two alkylated DNA strands. It appears that depurination events in one of the DNA strands of a phage particle are sufficient to cause death.