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J Virol. 1969 September; 4(3): 197-202
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Replication of Canine Herpesvirus

I. Synthesis of Viral Deoxyribonucleic Acid

Departments of Microbiology and Laboratory Animal Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205

ABSTRACT

This paper reports the results of two series of experiments. The first series indicated that deoxyribonucleic acid (DNA) extracted from partially purified canine herpesvirus virions is characterized by a high guanine plus cytosine molar base ratio (65 to 67 mole%), similar to the DNA of herpes simplex virus. In the second series of experiments it was estimated, on the basis of uptake of tritiated thymidine, that in dog kidney cells canine herpesvirus-DNA synthesis starts at 4 hr and continues until 16 hr after infection. Treatment of infected cells with puromycin during the first 4 hr of infection blocks the onset of viral DNA synthesis, whereas, after this time the uptake of thymidine is unaffected.