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J Virol. 1969 July; 4(1): 15-23
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Separation of a New Deoxyribonucleic Acid Polymerase from Vaccinia-infected HeLa Cells

K. I. Berns, C. Silverman and A. Weissbach1

Laboratory of Biology of Viruses, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20014

ABSTRACT

HeLa cell deoxyribonucleic acid (DNA) polymerase was purified about 100-fold by sequential column chromatography on phosphocellulose, hydroxylapatite, and Bio Rex 70. A new form of DNA polymerase found in vaccinia virus-infected cells was separated from HeLa DNA polymerase by chromatography on diethylamino-ethyl cellulose. The new form was also purified approximately 100-fold in the same manner as the HeLa DNA polymerase. In addition to chromatographic differences, the two enzymes differed with regard to primer response, relative activity at high pH, inactivation by heat and p-chloromercuribenzoate, and inhibition by vaccinia antiserum.

FOOTNOTES

1 Visiting Scientist, Roche Institute of Molecular Biology, Nutley, N.J.

J Virol. 1969 July; 4(1): 15-23
Copyright © 1969 American Society for Microbiology. All Rights Reserved.



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Copyright © 1969 by the American Society for Microbiology. All rights reserved.