This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by de Kinkelin, P Articles by Bernard, J Search for Related Content PubMed PubMed Citation Articles by de Kinkelin, P Articles by Bernard, J

 Previous Article  |  Next Article 

J Virol. 1980 December; 36(3): 652-658

Viral hemorrhagic septicemia of rainbow trout: selection of a thermoresistant virus variant and comparison of polypeptide synthesis with the wild-type virus strain.

ABSTRACT

Serial passage of viral hemorrhagic septicemia virus at gradually increasing temperature selected for a variant virus that replicates at 25 degrees C and has a low pathogenicity for rainbow trout. Viral hemorrhagic septicemia virus-specific polypeptide synthesis was examined in epithelioma papulosum cyprini cells infected with either a wild-type strain or a thermoresistant variant. The wild-type N and M1 proteins were synthesized throughout the course of infection, whereas L, G, and M2 were more actively translated later in the replication cycle. The wild-type strain was more cytotoxic at 25 than at 14 degrees C despite the fact that no translation could be evidenced when the temperature was raised. When epithelioma papulosum cyprini cells were infected with the variant virus, the kinetic study was obstructed since protein synthesis was difficult to observe by the pulse method at a low multiplicity of infection and aborted when the multiplicity of infection was raised. The variant was less cytotoxic at 25 degrees C than wild-type virus.

This article has been cited by other articles:

• Boudinot, P., Bernard, D., Boubekeur, S., Thoulouze, M.-I., Bremont, M., Benmansour, A. (2004). The glycoprotein of a fish rhabdovirus profiles the virus-specific T-cell repertoire in rainbow trout. J. Gen. Virol. 85: 3099-3108 [Abstract] [Full Text]