This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Villarreal, L P Articles by Jovanovich, S Search for Related Content PubMed PubMed Citation Articles by Villarreal, L P Articles by Jovanovich, S

 Previous Article  |  Next Article 

J Virol. 1980 November; 36(2): 595-600

Leakage of nuclear transcripts late in simian virus 40-infected CV1 cells: quantitation of spliced and unspliced late 19S RNAs.

ABSTRACT

During the late phase of simian virus 40 infections of CV1 cells, the relative ratios of the spliced to the unspliced RNA molecules of the 19S family were measured. In the cytoplasm, unspliced 19S RNA represented between 1 and 2% of spliced 19S RNA. This ratio could be altered by the use of different methods of RNA extraction such that unspliced RNA was observed at 10 to 20% of spliced RNA. The nuclear ratios of spliced to unspliced 19S RNA were also determined. In contrast to cytoplasmic RNA, nuclear unspliced RNA was several hundred percent that of nuclear spliced 19S RNA. Cytoplasmic unspliced 19S RNA appears to be of nuclear origin, and its presence in the cytoplasmic fraction is due to nuclear leakage during RNA fractionation.