This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Souza, L M Articles by Baluda, M A Search for Related Content PubMed PubMed Citation Articles by Souza, L M Articles by Baluda, M A

 Previous Article  |  Next Article 

J Virol. 1980 November; 36(2): 325-336

Identification of the avian myeloblastosis virus genome. II. Restriction endonuclease analysis of DNA from lambda proviral recombinants and leukemic myeoblast clones.

ABSTRACT

Two lambda proviral DNA recombinants were characterized with a number of restriction endonucleases. One recombinant contained a complete presumptive avian myeloblastosis virus (AMV) provirus flanked by cellular sequences on either side, and the second recombinant contained 85% of a myeloblastosis-associated virus type 1 (MAV-1)-like provirus with cellular sequences adjacent to the 5' end of the provirus. Comparing the restriction maps for the proviral DNAs contained in each lambda hybrid showed that the putative AMV and MAV-1-like genomes shared identical enzyme sites for 3.6 megadaltons beginning at the 5' termini of the proviruses with respect to viral RNA. Two enzyme sites near the 3'-end of the MAV-1-like provirus were not present in the putative AMV genome. We also examined a number of leukemic myeloblast clones for proviral content and cell-provirus integration sites. The presumptive AMV provirus was present in all the leukemic myeloblast clones regardless of the endogenous proviral content of the target cells or the AMV pseudotype used for conversion. Multiple cellular sites were suitable for integration of the putative AMV genome and the helper genomes. The proviral genomes were all integrated colinearly with respect to linear viral DNA.

This article has been cited by other articles:

• Shetzline, S. E., Rallapalli, R., Dowd, K. J., Zou, S., Nakata, Y., Swider, C. R., Kalota, A., Choi, J. K., Gewirtz, A. M. (2004). Neuromedin U: a Myb-regulated autocrine growth factor for human myeloid leukemias. Blood 104: 1833-1840 [Abstract] [Full Text]  
• Shen-Ong, G., Potter, M, Mushinski, J., Lavu, S, Reddy, E. (1984). Activation of the c-myb locus by viral insertional mutagenesis in plasmacytoid lymphosarcomas. Science 226: 1077-1080 [Abstract]  
• Mushinski, J., Potter, M, Bauer, SR, Reddy, E. (1983). DNA rearrangement and altered RNA expression of the c-myb oncogene in mouse plasmacytoid lymphosarcomas. Science 220: 795-798 [Abstract]  
• Rushlow, K., Lautenberger, J., Papas, T., Baluda, M., Perbal, B, Chirikjian, J., Reddy, E. (1982). Nucleotide sequence of the transforming gene of avian myeloblastosis virus. Science 216: 1421-1423 [Abstract]