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J Virol. 1980 May; 34(2): 532-541

Expression of Wound Tumor Virus Gene Products In Vivo and In Vitro

¹ Division of Laboratories and Research, New York State Department of Health, Albany, New York 12201

ABSTRACT

Infection of Agallia constricta vector cell monolayers with wound tumor virus results in the synthesis of 12 virus-specific polypeptides. Confirmation that these polypeptides are virus encoded rather than virus induced was obtained by cell-free translation of in vitro synthesized viral mRNA. In addition, transcription by purified wound tumor virus particles was coupled with translation of the resulting transcripts in a wheat embryo cell-free extract. Six previously described structural polypeptides, one presumptive structural polypeptide, and five previously unidentified nonstructural polypeptides were synthesized in infected vector cell monolayers, in cell-free extracts directed by in vitro synthesized viral mRNA, and in the homologous plant cell-free system, in which viral transcription was coupled with translation. Pulse-chase experiments revealed no evidence of precursor-product relationships for the wound tumor virus-specific polypeptides.