This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Chroboczek, J. Articles by Zagórski, W. Search for Related Content PubMed PubMed Citation Articles by Chroboczek, J. Articles by Zagórski, W.

 Previous Article  |  Next Article 

J Virol. 1980 May; 34(2): 330-335

Regulation of Brome Mosaic Virus Gene Expression by Restriction of Initiation of Protein Synthesis

Jadwiga Chroboczek, Ludmia Puchkova and Wodzimierz Zagórski

Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-532 Warsaw, Poland

ABSTRACT

The translation of total and individual brome mosaic virus (BMV) RNAs was examined in a wheat germ cell-free system in the presence of various inhibitors. Inhibitors of the initiation of polypeptide synthesis, e.g., potassium ions, 7-methylguanosine 5' -monophosphate, and aurintricarboxylic acid, were shown not only to inhibit overall BMV protein synthesis but also to change the ratio of BMV polypeptides synthesized. Under conditions restrictive for initiation, the translation of nonstructural BMV genes was suppressed, but coat protein synthesis proceeded at a high rate. A similar discrimination among BMV messengers was exerted by a regulatory protein kinase isolated from wheat germ. These results suggest that the regulation of the expression of BMV genes is based on a difference in the mechanism of formation of initiation complexes for individual BMV messages.

Permanent address: Institute of Experimental Medicine, 197022 Leningrad, USSR.