This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Martin, R G Articles by Edwards, C A Search for Related Content PubMed PubMed Citation Articles by Martin, R G Articles by Edwards, C A

 Previous Article  |  Next Article 

J Virol. 1979 September; 31(3): 596-607

Roles of the simian virus 40 tumor antigens in transformation of Chinese hamster lung cells: studies with simian virus 40 double mutants.

ABSTRACT

Simian virus 40 mutants containing both a tsA mutation (rendering the 90,000 molecular weight [90K] T-antigen thermolabile) and a deletion between 0.54 and 0.59 map units (reducing the size and the amount of the 20K t-antigen) were used to transform Chinese hamster lung cells. The frequencies of transformation by the double mutants were comparable to that of the tsA mutant alone by both the focus and agar assays except when the cells were serum depleted before infection. Growth-arrested cells were transformed (using the agar assay) by the deletion mutants at less than 2% the frequency found when the 20K t-antigen was normal. Growth arrest had very little effect on the temperature sensitivity of the resultant transformed cell lines whether or not the deletion was present.

This article has been cited by other articles:

• Mateer, S. C., Fedorov, S. A., Mumby, M. C. (1998). Identification of Structural Elements Involved in the Interaction of Simian Virus 40 Small Tumor Antigen with Protein Phosphatase 2A. J. Biol. Chem. 273: 35339-35346 [Abstract] [Full Text]